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植物外源基因拷贝数及插入位点的检测方法与技术 被引量:8

Methods and Techniques for Estimating the Copy Number and Flanking Sequences of Exogenous Gene in Transgenic Plants
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摘要 随着转基因技术的快速发展,转基因植物在世界范围内已经大量种植.近年来,转基因植物的安全性越来越受到公众关注.转基因植物安全性与有效性的关键因素是外源基因拷贝数与插入位点.本文综述了近年来植物外源基因拷贝数与插入位点的检测方法. With the rapid development of plant transgenic technologies and the worldwide cultivation of transgenic plants, more considerations were shown to the safety of transgenic plants. The copy number of exogenous genes and flanking sequences were indispensable for the safety and effectiveness of transgenie plants. In this paper, methods and techniques for estimation the copy number of exogenous genes and flanking sequences in transgenic plants had been reviewed.
出处 《河南师范大学学报(自然科学版)》 CAS CSCD 北大核心 2012年第6期111-116,共6页 Journal of Henan Normal University(Natural Science Edition)
关键词 转基因植物 外源基因 拷贝数 插入位点 transgenic plants exogenous gene copy number flanking sequences
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  • 1曾凡锁,詹亚光.转基因植物中外源基因的整合特性及其研究策略[J].植物学通报,2004,21(5):565-577. 被引量:23
  • 2Cervera M,Pina J A,Juárez J,et al.Broad exploration of a transgenic population of citrus:stability of gene expression and phenotype[J].Theor Appl Genet,2000,100:670-677.
  • 3Dominguez A,Guerri J,Cambra M,et al.Efficient production of transgenic citrus plants expressing the coat protein gene of citrus tristezavirus[J].Plant Cell Rep,2000,19:427-433.
  • 4Chen J,Carlson A R,Wan J,et al.Chromosomal location and expression of green fluorescent protein(gfp)gene in microspore derivedtransgenic barley(Hordeum vulgare L.)[J].Acta Genetica Sinica,2003,30(8):697-705.
  • 5Tang J L,Scarth R,Fristensky B.Effects of genomic position and copy number of Acyl-ACP thioesterase Transgenes on the level of thetarget fatty acids in Brassica napus L[J].Molecular Breeding,2003,12:71-81.
  • 6Taline E,Florence P,HervéV.Arabidopsis RPA2:A Genetic Link among Transcriptional Gene Silencing,DNARepair,andDNAReplica-tion[J].Current Biology,2005,25(21):1919-1925.
  • 7蒋自立.植物DNA甲基化与转基因沉默[J].安徽农业科学,2009,37(12):5386-5389. 被引量:6
  • 8Choi H W,Lemaux P G,Cho M J.Use of fluorescence in situ hybridization for gross mapping of transgenes nd screening for homozygousplants in transgenic barley(Hordeum vulgare L.)[J].Theor Appl Genet,2002,106(1):92-100.
  • 9Brunaud V,Balzergue S,Dubreucq B.T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites[J].EMBO Rep,2002,3(12):1152-1157.
  • 10Sallaud C,Gay C,Larmande P,et al.High throughput T-DNA insertion mutagenesis in rice:a first step towards in silico reverse genet-ics[J].Plant Journal,2004,39:450-464.

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