摘要
目的研究PLGA(poly lactide-co-glycolide)-胶原复合支架上大鼠成肌细胞体外张应力作用下整合素偶联激酶(ILK)的表达变化,探索其力学信号转导过程。方法体外培养大鼠成肌细胞,实验组接种于黏附有PLGA-胶原复合生物支架的加力板,对照组接种于空白加力板,然后使用"Forcel"四点弯曲循环应变加载装置加载。利用RT-PCR技术,检测不同时间点细胞ILK mRNA的表达,比较三维PLGA-胶原复合支架上和二维平面上培养的细胞在张应力作用下基因水平的表达差异。结果张应力刺激下,同一时间点三维支架上细胞ILK的表达明显高于二维平面上的细胞;在加载过程中,三维支架上细胞ILK的表达水平明显提高,而且更快达到峰值。结论培养环境的三维空间结构对细胞在力学刺激作用下的初期反应起重要作用。
Objective To study the expression changes of rat myoblasts cultured in vitro in a PLGA-collagen scaffold after mechanical stimulation in order to better understand the mechanotransdution process. Methods Rat myoblasts were cultured in vitro. Then those in the experimental group were vaccinated on the plane adhered to PLGA-collagen scaffold while those in the control group were vaccinated on a plane. A cyclic strain loading apparatus, ＂Forcel＂ four-point bending device, was used for mechanical loading. The post-stress expression of ILK mRNA was tested by RT-PCR. The difference in the expression of cells cultured in a three-dimensional PLGA- collagen scaffold and that on a two-dimensional surface at the gene level was compared. Results Under the tension stress, myoblasts cultured in the three-dimensional system had a greater expression than that on the twodimensional surface at the same time point ; the expression of the cells in three-dimensional scaffold reached the peak faster than that on the two-dimensional surface. Conclusion The ILK mRNA expression of the cells is greater in a three-dimensional scaffold than on a two-dimensional surface. Therefore, it can be concluded that the local architecture conditions play an important role in ceils＇ initial responses to mechanical stimuli.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期46-49,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省自然科学基金资助项目(No.2009JM4026)~~
