摘要
对金钱白花蛇进行几种凝胶电泳研究 ,根据聚丙烯酰胺凝胶电泳 (PAGE)谱带的位置和数目进行品种鉴别 ;用改进的十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳 (SDS- PAGE)测定其主要蛋白质成分分子量 ;用等电聚焦电泳(IFE)测定其主要蛋白质成分的等电点 (PI)。清晰的电泳谱带及相应数据为商品蛇真伪鉴别提供了科学依据。
Disc polyacrylamide gel electrophoresis(PAGE)was performed to detect systematically the soluble proteins in Bungarus. Different species were identified according to the position and number of PAGE bonds.Molecular weights of the soluble proteins were measured by a modified sodium dodecyl sulfonate (SDS) procedure. Isoelectric focusing electrophoresis (IFE) was used to determine the isoelectric point of the main protein of Bungarus. Results showed that the IFE band were always quite distict, which can be used as a scientific basis to distinguish the genuine snakes from its confusable or faked varieties.
出处
《中草药》
CAS
CSCD
北大核心
2000年第5期374-377,共4页
Chinese Traditional and Herbal Drugs
基金
湖北省教委自然科学科研项目
关键词
金钱白花蛇
可溶性蛋白
凝胶电泳图谱
IFE
Bungarus polyacrylamide gel electrophoresis (PAGE) sodium dodecyl sulfonate (SDS) isoelectric focusing electrophoresis (IFE)
