摘要
目的:建立各方面更接近人类的小细胞肺癌(SCLC)动物模型,为SCLC的基础和临床研究提供参考。方法:以非人灵长类动物食蟹猴作为实验动物,经切除脾脏后,通过支气管镜直接注入人类SCLC细胞株Nci-h446至食蟹猴的支气管黏膜,诱导建立人SCLC的食蟹猴模型。通过胸部CT扫描和经支气管镜活检送病理,鉴定模型建立的成功率。结果:人小细胞肺癌食蟹猴模型的建立所需时间最少需要9个月,但最早约6个月即能发现细胞水平的变化(核异质变)。食蟹猴模型的生活状态、生化水平及影像学改变都与人类肺癌的发病过程相似。6只食蟹猴成功生长出肺癌的有3只,模型建立成功率为50%。按接种部位计算,12个接种细胞浓度为1×107 mL-1的位点,成功生长出肺癌的3个,成功率为16.7%;接种细胞浓度为1×107 mL-1的位点,出现细胞核异质以上改变(包括癌变)的5个,占41.7%;但接种细胞浓度为1×105 mL-1和接种细胞浓度为1×106 mL-1的位点,成功率为0。所有36个接种位点成功率为8.3%,出现细胞核异质以上改变(包括癌变)的百分率为13.9%。结论:通过支气管镜直接接种人类SCLC细胞株Nci-h446至食蟹猴的支气管黏膜,能成功建立人SCLC的食蟹猴模型,但细胞浓度至少达1×107 mL-1。
OBJECTIVE： To set up SCLC animal model which was similar to human beings. METHODS： Macaca fascicu laris were used as in vivo model. Macaca fasciculari model of SCLC was induced by directly injecting human SCLC cell lines Nci h446 into the bronchial mucosa through bronchoscopy after spleen resection. This model was successfully established and deter mined by chest CT scan and bronchoscopy biopsy. RESULTS：The time of a successful macaca fasciculari model of SCLC needed at least 9 months, but the earliest subject showed pathological changes of dyskaryosis was about 6 months. The life state and bio chmical level and imaging change of the macaca fasciculari model of SCLC was similar to that of human lung cancer. The success ful rate of SCLC model was 50 %, namely 3 in 6 macaca fascicularis. In the 12 sites injected with 1 × 107 cells mL-1 , the success ful rate was 16.7% ,namely 3 in 12 sites; while for those injection with 1 × l0s or 1 × 106 ceils mL-1 ,all failed. Among those sites implanted with 1 × 107 ceils mL-1 ,41.7% subjects （5 sites） showed pathological changes of dyskaryosis or worse （including canceration）. The average percentage of successful establishment for all sites injected with cells was 8.3 %; 13.9 %sites showed pathological changes of dyskaryosis or worse （including canceration） in all sites. CONCLUSION： Macaca fascicularis model of SCLC can be induced by directly injecting human SCLC cell lines Nci-h446 into the animal＇s bronchial mucosa through bronchos- copy,which requires the cell concentration more than 1 × 107 mL 1.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第23期1776-1779,1840,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省科技计划(2010B031600034)
关键词
癌
小细胞肺
食蟹猴
疾病模型
动物
carcinoma, small cell lung macaca fascicularis
disease models, animal
