摘要
目的:检测妊娠期糖尿病(GDM)孕妇血清及脐血血清中晚期糖基化终产物(AGE)的水平,并观察胎盘组织中AGE受体(RAGE)的表达,探讨AGE与胎儿出生缺陷的关系及其在产前筛查中的临床意义。方法:选择经产前筛查诊断为胎儿畸形或胎死宫内的GDM孕妇作为病例组(42例),随机选择GDM无胎儿异常孕妇作为GDM组(30例),无妊娠合并症的健康孕妇作为健康对照组(30例)。采用酶联免疫法检测孕妇血清及脐血血清AGE水平;采用免疫组化方法检测胎盘组织中RAGE蛋白的表达。结果:病例组、GDM组、健康对照组脐血血清AGE水平分别为(223.9±54.6),(160.7±37.2),(108.3±15.8)μg/L,三组差异显著(P<0.05);病例组、GDM组、健康对照组孕妇血清AGE水平分别为(169.1±14.3),(105.4±17.2),(80.6±11.4)μg/L,三组亦有显著差异(P<0.05);病例组孕妇血清与脐血血清中AGE水平呈正相关(r=0.863,P<0.01);病例组、GDM组及健康对照组胎盘组织中RAGE的阳性表达率分别为87.5%、53.1%、29.6%,三组差异显著(P<0.05)。结论:GDM孕妇高AGE血症是导致出生缺陷的危险因素,孕妇血清AGE水平可作为GDM出生缺陷的产前筛查指标。
To detect serum advanced glycation end products（AGE） level in gestation diabetic mother（GDM） gestational period and fetal cord blood,and observe the RAGE protein expression in placenta.To explore the clinical significance of relationship between fetal birth defects and AGE levels in prenatal screening.Methods：Gestational diabetes pregnant woman confirmed fetalmalformation or fetal death by prenatal diagnosis were enrolled as the study group,total of 42 cases;Randomly selected the GDM fetal normalities pregnant women,as the GDM group,30 cases;another 30 cases of the normal pregnant women wre selected randomly as healthy control group.Maternal peripheral blood and the specimens of fetal cord blood specimens were collected to detect AGE by enzymatic assay.The line RAGE immunohistochemistry.Results：The case group,the GDM group and healthy control group fetal cord blood serum AGE levels were（223.9±54.6）,（160.7±37.2）,（108.3±15.8）μg/L,Comparison of the difference were statistically significant（P0.05）.The case group,the GDM group and healthy control group maternal serum AGE levels were（169.1±14.3）,（105.4±17.2）,（80.6±11.4）μg/L,comparison of the difference were statistically significant（P0.05）.The case group,the GDM group and healthy control group the RAGE protein positive expression rate in placental tissue were 87.5%、53.1%、29.6%,the difference were statistically significant（P0.05）.Conclusions：GDM maternal AGE hyperlipidemia is the risk factors for fetus birth defects,Pregnant women serum AGE levels can be used as GDM birth defects of prenatal screening index
出处
《现代妇产科进展》
CSCD
2012年第12期917-920,共4页
Progress in Obstetrics and Gynecology
基金
2011年度广州市卫生局医药卫生科技项目
一般引导项目(No:201102A213037)
关键词
晚期糖基化终产物
妊娠期糖尿病
胎儿
先天畸形
产前诊断
胎盘
血清
Advanced glycosylation end products
Gestation diabetes mellitus
Fetus
Congenital abnormalities
Prenatal diagnosis
Placenta
Serum
