摘要
目的:研究牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)上清液对人脐静脉内皮细胞(hu-man umbilical vein endothelial cells,HUVECs)分泌IL-1β、IL-6的影响。方法:酶消化法原代培养HUVECs,并对细胞进行来源鉴定,用5×106CFU/mL的Pg上清液刺激HUVECs 6、12、18、24 h后,RT-PCR检测IL-1β、IL-6 mRNA的表达;同时ELISA方法测定IL-1β及IL-6蛋白的水平。结果:Pg上清液刺激HUVECs后,IL-1βmRNA水平在12、18 h时蛋白明显高于对照组(P<0.05);IL-6 mRNA水平在12 h的表达明显高于对照组(P<0.05)。IL-1β蛋白在18、24 h的表达明显高于对照组(P<0.05);IL-6蛋白在12、18、24 h 3个时点均明显高于对照组(P<0.05)。结论:5×106CFU/mL Pg上清液可促进人脐静脉内皮细胞IL-1β和IL-6的基因及蛋白表达,提示Pg感染可能在As的发生发展中起一定作用。
AIM： To observe the effects of Porphyromonas gingivalis on expression of IL-6 and IL-1β in human umbilical vein endothelial cells （HUVECs）. METHODS： Primary clutures of HUVECs were derived by en- zyme digestion method. Passage 5 HUVECs were stimulated by P. gingivalis supernatant for 6, 12, 18 and 24 h. At specific time points, mRNA was extracted and subjected for RT-PCR to detect IL-6 and IL-1β mRNA expression. HUVEC culture supernatant was collected and subjected for ELISA to measure IL-6 and IL-1β protein levels. RESULTS： P. gingivalis supernatant stimulation significantly increased IL-1β mRNA expression at 12 and 18 h （P 〈0.05）, and it also significantly increased IL-6 mRNA at 12 h （P 〈0.05）. Furthermore, P. gingivalis stimula- tion resulted in significantly higher IL-1βsecretion at 18 and 24 h （ P 〈 0.05 ）. And IL-6 protein level was significant- ly augmented at 12, 18 and 24 h （P 〈 0.05）. CONCLUSION ： P. gingivalis can stimulate the expression of IL-6 and IL- 1β in human umbilical vein endothelial cells.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2012年第12期709-712,共4页
Chinese Journal of Conservative Dentistry
基金
云南省科技厅省校联合专项资助项目(2008CD057)
云南省科技厅科技计划资助项目(2010ZC115)
