摘要
目的:研究不同浓度的三氧化矿物凝聚体(mineral trioxide aggregate,MTA)对成人牙髓干细胞(human dental pulp stem cells,hDPSCs)增殖和分化的影响。方法:体外培养hDPSCs,分别以不同浓度MTA浸提液作用于hDPSCs。采用四甲基偶氮噻唑蓝(methyl thiazoly tetrazolium,MTT)法测定MTA对hDPSCs增殖的影响;Von Kossa染色法检测hDPSCs钙化结节的形成;通过酶标仪法检测hDPSCs碱性磷酸酶(alkalinephosphatase,ALP)的活性;运用逆转录聚合酶链反应(RT-PCR)法检测牙本质涎磷蛋白(dentin sialophosphopro-tein,DSPP)基因的表达。采用单因素方差分析对数据进行统计学分析。结果:2 mg/mL MTA能促进hDPSCs增殖,其ALP活性也明显高于其他各组,并可见Von Kossa染色呈阳性,有钙化结节形成,且能明显促进牙髓干细胞表达DSPP;而20 mg/mL MTA则抑制hDPSCs的增殖,ALP活性明显低于其他各组,Von Kossa染色阴性;而0.2 mg/mL MTA组及氢氧化钙组未能表现出明显的增殖分化活性。结论:2 mg/mL MTA能促进hDPSCs的增殖,并能诱导hDPSCs向成牙本质细胞方向分化。20 mg/mL MTA抑制hDPSCs的增殖,并且抑制hDPSCs向成牙本质细胞方向分化。
AIM： To investigate the effects of mineral trioxide aggregate （MTA） on the proliferation and differentiation of human dental pulp stem cells （hDPSCs）. METHODS： hDPSCs of 4 -9 passages were used in the study, hDPSCs were cultured with different concentrations of MTA leaching solutions. Cell proliferation was detected by MTY array. Von Kossa staining was employed to observe the formation of mineralized nodules. The activity of alkaline phosphatase （ALP） was determined by the microplate method. The mRNA expression of dentin sialophosphoprotein （DSPP） was determined by RT-PCR. ANOVA was employed to analyze the results. RESULTS： The 2mg/mL MTA group proliferated significantly faster than the other groups, while 20mg/mL MTA group grew slower than the that on control significantly. The formation of mineralized nodules was found in 2mg/mL MTA group, but not found in other groups. The activity of ALP in 2mg/mL MTA group was higher than that on other groups ,while the activity of ALP in 20mg/mL MTA was slower than that on control group. The mRNA expression of DSPP was discovered in all groups. DSPP expression in 2mg/mL MTA group was significantly stronger than that on the other two groups. CONCLUSION： 2 mg/mL MTA can promote the proliferation of hDPSCs and induce hDPSCs to differentiate into odontoblast cells. 20mg/mL MTA can inhibit the proliferation of hDPSCs and prevent hDPSCs to differentiate into odontoblast cells.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2012年第12期690-694,共5页
Chinese Journal of Conservative Dentistry
关键词
三氧化矿物凝聚体
牙髓干细胞
碱性磷酸酶
牙本质涎磷蛋白
mineral trioxide aggregate
human dental pulp stem cells
alkaline phosphatase
dentin sialophos-phoprotein
