摘要
目的观察FoxO3a基因缺失致小鼠脾脏进行性慢性炎性反应。方法分别于16周、24周和12个月称取FoxO3a基因敲除小鼠和对照小鼠体重;于16、24和38周处死各组小鼠,取脾脏,肉眼观察脾脏变化,并制备脾细胞悬液,进行细胞计数,同时取各组小鼠卵巢、肺及皮下等组织,观察其炎性变化。流式细胞术检测24周的FoxO3a基因敲除小鼠和对照小鼠脾细胞中T、B淋巴细胞和Mac-1+细胞数量及百分率;Real-time PCR检测脾细胞中炎性细胞因子S100A8和S100A9基因mRNA的表达水平。结果与对照小鼠相比,FoxO3a基因敲除小鼠脾脏明显肥大,且随年龄增加更加显著,卵巢、肺及皮下等组织内可见大量炎性细胞浸润,脾细胞数明显升高;基因敲除组老年小鼠的体重明显低于对照小鼠;FoxO3a基因敲除小鼠的脾细胞中T、B淋巴细胞数增加明显,但所占脾细胞的百分率无明显增加,Mac-1+细胞数和百分率均明显增加;S100A8和S100A9基因mRNA的表达水平明显高于对照小鼠。结论 FoxO3a基因缺失致小鼠脾脏进行性慢性炎性反应。
Objective To observe the progressive chronic inflammatory reaction induced by FoxO3a gene knockout in mice.Methods The bodyweights of FoxO3a gene knockout and normal control mice were weighed at ages of 16 weeks,24 weeks and 12 months separately.Partial mice in the two groups were killed at ages of 16,24 and 38 weeks respectively,of which the spleens were collected,observed visually,prepared into splenocytes and counted.Meanwhile,the inflammatory reactions in various tissues including ovary,lung and subcutaneous tissues were observed.The counts and percentages of T and B lymphocytes and Mac-1＋ cells in spleens of FoxO3a gene knockout and control mice at age of 24 weeks were determined by flow cytometry,while the expression levels of S100A8 and S100A9 mRNAs in splenocytes by real-time PCR.Results Compared with those of control mice,the spleens of FoxO3a gene knockout mice were enlarged significantly,which were age-dependent,while the infiltration of a large quantity of inflammatory cells were observed in ovary,lung and subcutaneous tissues,and the counts of splenocytes as well as T and B lymphocytes increased significantly.However,the percentages of T and B lymphocytes in splenocytes showed no significant increase.Both the count and percentage of Mac-1＋ cells in FoxO3a gene knockout mice increased significantly,while the expression levels of S100A8 and S100A9 mRNAs were significantly higher than those in control mice.The bodyweights of aged FoxO3a gene knockout mice were significantly lower than those of aged control mice.Conclusion FoxO3a gene knockout induced progressive chronic inflammatory reaction in spleens of mice.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第12期1650-1652,1657,共4页
Chinese Journal of Biologicals
基金
国家教育部留学归国启动基金
辽宁省教育厅高校科研项目(2009A196)
教育部科技发展中心博士点专项科研基金(20112105120009)
