摘要
以梭梭无菌苗和肉苁蓉萌发种子为材料,对肉苁蓉离体寄生培养基质、离体寄生方式和离体寄生环境条件等进行了研究,以明确肉苁蓉的寄生特性,为肉苁蓉人工繁殖技术体系的建立与优化奠定基础。结果表明:玻璃纤维滤纸是肉苁蓉离体寄生适宜的培养基质;在黑暗、相对湿度80%、温度18℃～30℃的环境中,萌发的肉苁蓉种子与寄主梭梭寄生培养8d后开始建立寄生关系,寄生率达82%;而光照条件下,萌发的肉苁蓉种子与梭梭不能建立寄生关系。
In order to identify parasitic property and to provide foundation for construction and optimization of manual propagation technology of Cistanche deserticola ,we studied the medium, method and surround- ings condition of in vitro parasitism with aseptic seeding of Haloxylon ammodendron and germinate seed of C. deserticola. The results showed that glass fiber filter was suitable culture medium of in vitro system par- asitism of C. deserticola. Under condition of dark,relative humidity at 80% and temperature between 18℃ ～30℃ ,germinated seed of C. deserticola parasite H. ammodendron for 8 days,and the highest parasitism rate was 82 %. But germinated seed could not parasite H. arnmodendron under light condition.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第12期2556-2559,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30960023和31160066)
宁夏自然科学基金(NZ12262)
关键词
肉苁蓉
人工控制
离体寄生
寄生率
Cistanche deserticola
manual control
in vitro parasitism
parasitism rate
