摘要
目的 观察人重组胰岛素样生长因子 1(rhIGF 1)对糖尿病大鼠肾脏的影响。方法 采用生化法、放射免疫法及逆转录 聚合酶链反应 (RT PCR)技术等方法。结果 (1)糖尿病治疗组 2 4h尿白蛋白排泄率和尿量低于糖尿病对照组 ;(2 )糖尿病对照组血清IGF 1含量、肾组织IGF 1含量及IGF 1信使核糖核酸 (IGF 1mR NA)表达明显低于正常对照组 ;糖尿病治疗组血清IGF 1含量明显高于糖尿病对照组 ,肾组织IGF 1含量及IGF 1mRNA表达与糖尿病对照组无明显差异 ;(3)糖尿病对照组血清生长激素 (GH)高于正常对照组 ,糖尿病治疗组血清GH低于糖尿病对照组 ;(4)电镜观察大鼠肾脏病理切片发现rhIGF 1对糖尿病大鼠肾脏损害有改善作用。结论 小剂量外源性rhIGF 1不会加重糖尿病肾脏的损害。
Purpose To observe the effects of rhIGF?1 on kidney in diabetic rats. Methods Using biochemistry,radioimmunoassay,molecular biology (RT?PCR). Results (1) 24 h UAER,24 h uriary volume in rhIGF?1 group is lower than that of diabetic control group;(2) The level of serum IGF?1,kidney IGF?1 and IGF?1 mRNA in diabetic control group is lower than that of normal control group;The level of serum IGF?1 in rhIGF?1 group is higher than that of diabetic control group;no differences were found in the levels of kidney IGF?1 and kidney IGF?1 mRNA between diabetic control group and rhIGF?1 group;(3) The level of serum GH in diabetic control group is higher than that of normal control group;The level of serum GH in rhIGF?1 group is lower than that of diabetic control group;(4) rhIGF?1 might have some protective effects on diabetic nephropathy via electron microscope. Conclusions rhIGF?1 doesn't increase the damage of diabetic kidney tissue.
出处
《上海医科大学学报》
CSCD
2000年第3期197-200,221,共5页
Journal of Fudan University(Medical Science)
