摘要
:目的 研究脂质体瘤苗激活小鼠巨噬细胞的过继免疫治疗作用。方法 制备脂质体 H2 2 肝癌瘤苗 ,脂质体 S1 80 瘤苗 ,Freund's完全佐剂 H2 2 肝癌瘤苗以及对照脂质体瘤苗并免疫小鼠。用 Hanks液清洗免疫鼠的腹腔收集糖原诱导的腹腔巨噬细胞。实验鼠腹腔注射 1× 10 5 个 H2 2 肝癌细胞 2 4小时后再腹腔给予各供体巨噬细胞 5× 10 6个 /只。结果 脂质体 H2 2 瘤苗和 Freund's佐剂 H2 2 瘤苗与对照组相比较 ,其免疫鼠提供的巨噬细胞具有较强的过继免疫治疗作用。结论 脂质体瘤苗激活的小鼠巨噬细胞具有特异性的可过继转移的抗瘤作用。
Objective To study the antitumor effect of adoptive immunotherapy of using murine macrophages activated with liposome tumor vaccine.Methods Liposome H 22 hepatic cancer vaccine,liposome S 180 vaccine,Freund's complete adjuvant H 22 hepatic cancer vaccine and liposome control were prepared and immunized mice respetively.Immune murine macrophages induced by glycogen were harvested by washing the peritoneal cavities with Hank's balanced salt solution.Each recipient animal was given an injection i.p. of 1×10 5 H 22 cells and was subsequently treated with 5×10 6 macrophages i,p.variens immuned mice at 24 h after tumor cell inoculation.Result The macrophages offered by mice immunized with liposome H 22 vaccine and Freund's complete adjuvant H 22 vaccine had better effect than those by control mice.Conclusion The murine macrophages activated with liposome tumor vaccine had specific adoptive transfer antitumour effect.
出处
《实用肿瘤杂志》
CAS
北大核心
2000年第3期162-164,共3页
Journal of Practical Oncology
关键词
过继免疫疗法
巨噬细胞
脂质体
肝肿瘤
immunotherapy,adoptive
macrophage
hepatic cancer H 22 /immunology
liposomes
liver neoplasms,experimental
