摘要
目的：研究肝细胞癌（ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ，ＨＣＣ）多药耐药（ｍｕｔｉｄｒｕｇ ｒｅｓｉｓｔａｎｃｅ，ＭＤＲ）基因表达蛋白Ｐ－糖蛋白（Ｐ－ｇｌｙｃｏｐｒｏｔｅｉｎ，Ｐ－ｇｐ））与转移能力的关系。方法：用链霉亲合素－生物素酶复合物（ｓｔｒｅｐｔ ａｖｉｄｉｎ－ｂｉｏｔｉｎｃｏｍｐｌｅｘ，ＳＡＢＣ ）免疫组化方法，检测５７例ＨＣＣ标本中Ｐ－ｇｐ，ｎｍ２３／ＮＤＰＫ，ｒａｓ／ｐ２１，Ｌａｍｉｎｉｎ（ＬＮ），Ｅ－ｃａｄ的含量。结果：Ｐ－ｇｐ阳性百分数为 ５２．６％． Ｐ－ｇｐ阳性和阴性表达者ｎｍ２３阳性白分数分别为４３． ３％和 ７７.７％（ Ｘ~２＝７１． Ｐ＜ ０. ０１），ｒａｓ阳性百分数分别为７６.７％和４４.４％（Ｘ~2＝６.２３，ｐ＜０.０５），ＬＮ阳性百分数分别为２３.３％和３３.３％（Ｘ２＝０.７， ｐ＞ ０.０５）， Ｅ-ｃａｄ阳性百分数分别为 ６０％和 ４４.４％（Ｘ~２＝１.３８， Ｐ＞ ０.０５），侵袭转移率分别为 ７０.０％和４０.７％（Ｘ~2＝４.９４， Ｐ＜ ０.０５）。结论：肝细胞癌Ｐ－ｇｐ高表达与癌细胞侵袭能力增强相关；与ｎｍ２３、ｒａｓ癌基因表达改变有关。
Objective: Whether the phenotypes of drug resistance and metastatic activity in cancer are dependent on each other or not is controversial. We studied the relationship between P-glycoprotein and metastatie activity in hepatocellular carcinoma (HCC). Methods: Fifty-seven histologically histologically verified HCC specimens were of obtained. SABC immunohistochemical staining with monoclonal antibody against human P-gp,, E-cad, Laminin, and with multiclonal antiboly against human NDPK, ras /p21. respectively, were usd to observe the P-gp, E-cad,Laminin, NDPK, p21 in all specimens. Results: nm23 /NDPK, ras/p21, Laminin and E-cad positive rate in P-gp(+) specimens were 43. 3%. 76.7%. 23.3% and 60%. while in P-gp(-), were 77.7%. 44.4%. 33.3% and 44.4%, respectively(x^2 value was 7.1. 6. 23, 0.7 and 1.38, P, value was <0.01. < 0.05, > 0.05 and > 0.05, respectively).The incidence of invasiveness and metastasis in P-gp(+) was 70. 0%. in p-gp (-) was 40. 7% (X2=4.94, p < 0.05 ). Conclusion:Our data suggest that overexpression of P-gp in HCC may covariate with the phenotypes of both enhanced metastasis and low nm23 expression and high ras expression.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第6期561-564,共4页
Chinese Journal of Cancer
