摘要
从浙江温州某制革厂污泥中筛选到1株细菌,对其形态和16S rDNA序列研究表明,细菌细胞呈杆状,革兰氏染色阴性,其16S rDNA序列与嗜酸性氧化亚铁硫杆菌标准菌株Acidithiobacillus ferrooxidans ATCC23270的序列相似度为99%,可鉴定为嗜酸性氧化亚铁硫杆菌菌株,暂命名为WZ-1.为了研究电镀污泥浸出液中重金属离子对WZ-1活性的影响,考察了Ni2+、Cr3+、Cu2+、Zn2+这4种重金属离子及5种不同模拟电镀污泥重金属浸出液对WZ-1 Fe2+氧化活性和表观呼吸速率的影响,结果表明菌株在接种量6.7%、初始pH 2.0、温度30℃、转速150 r·min-1的条件下,浓度分别为5.0 g·L-1、0.1 g·L-1的Ni2+、Cr3+对WZ-1的活性没有影响;WZ-1对Ni2+、Cr3+、Cu2+、Zn2+的最大耐受浓度分别为:30.0、0.1、2.5、30.0 g·L-1;5种模拟电镀污泥重金属浸出液对WZ-1活性的影响有明显差异,抑制强弱顺序为:Cu/Ni/Cr/Zn>Cu/Ni/Zn>Cu/Cr/Zn>Cu/Ni/Cr>Ni/Cr/Zn.
An Acidithiobacillus ferrooxidans strain WZ-1 was isolated from the tannery sludge in Wenzhou,Zhejiang Province in China.The cell of WZ-1 strain is Gram negative and rod-shaped,its 16S rDNA sequence is closely related to that of Acidithiobacillus ferrooxidans ATCC23270 with 99% similarity.These results reveal that WZ-1 is a strain of Acidithiobacillus ferrooxidans.The effects of Ni2＋,Cr3＋,Cu2＋,Zn2＋ and 5 kinds of simulated leaching solutions of electroplating sludge on the bioactivity of Fe2＋ oxidation and apparent respiratory rate of WZ-1 were investigated.The results showed that Ni2＋ and Cr3＋ did not have any influence on the bioactivity of WZ-1 at concentrations of 5.0 g·L-1 and 0.1 g·L-1,respectively.WZ-1 showed tolerance to high levels of Ni2＋,Zn2＋（about 30.0 g·L-1）,but it had lower tolerance to Cr3＋and Cu2＋（0.1 g·L-1Cr3＋and 2.5 g·L-1 Cu2＋）.Different kinds of simulated leaching solution of electroplating sludge had significant differences in terms of their effects on the bioactivity of WZ-1 with a sequence of Cu/Ni/Cr/ZnCu/Ni/ZnCu/Cr/ZnCu/Ni/CrNi/Cr/Zn.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2013年第1期209-216,共8页
Environmental Science
基金
浙江省重大科技专项(2010C03003)
关键词
嗜酸性氧化亚铁硫杆菌
活性
表观呼吸速率
电镀污泥重金属浸出液
Acidithiobacillus ferrooxidans
bioactivity
apparent respiratory rate
simulated heavy metal leaching solution of electroplating sludge
