摘要
目的制备专门针对富含亮氨酸重复区免疫球蛋白样蛋白1(LRIG1)胞外段(或称可溶性LRIG1,sLRIG1)抗体。方法人工设计、合成sLRIG1抗原多肽,免疫日本大耳朵白兔,颈动脉取血,分离兔血清;酶联免疫吸附分析法测定抗体效价,免疫印迹鉴定所获抗体。结果抗sLRTG1抗体滴度可高达1:512000;免疫印迹证实所获抗体能与sLRIG1特异性结合,在100kD处蛋白显影清晰。结论成功获得兔抗人sLRIG1多克隆抗体,可满足进一步研究的需要。
Objective To obtain the good quality antibody to the soluble leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1）. Methods The ectodomains, i.e. soluble LRIG1 （gene No. AF381545）, which were designed and synthesized in vitro, were intracutaneously injected in Japanese white rabbits. The antibodies which were obtained from the immunized rabbits＇ serum were purified. The obtained antibodies were identified by SDS-PAGE and western blotting. The titre of the antibodies was measured by enzyme-linked immunoassay. Results The antibodies which could specifically bind to the soluble LRIG1 were successfully^obtained. There was a clear protein blot around 100 kD on the western film as expected. The titre of the antibodies was high as 1 ： 512 000. Conclusion The polyclonal antibodies to soluble LRIG1 which were successfully obtained may satisfy the needs of the further experimental research.
出处
《中国临床神经外科杂志》
2012年第12期743-745,共3页
Chinese Journal of Clinical Neurosurgery
基金
国家自然科学基金资助项目(30973073
81172402)
教育部新教师基金(200804861039)
