摘要
目的:观察苦豆子总碱(total alkali sophora alopecuroids L,TASa)对大鼠胃窦环形肌条的作用及机制。方法:采用离体恒温灌流浴槽,记录胃窦环形肌条收缩波的振幅、持续时间、收缩面积和频率,给予TASa(5、10、20、40、80mg/L)观察其量效关系(r);将标本分为对照组(NS)、TASa、TASa+阿托品(10-6mg/L)、TASa+酚妥拉明(10-6 mg/L)、TASa+苯海拉明(10-6 mg/L)、TASa+消炎痛(10-6 mg/L)和TASa+异搏定(10-7 mg/L)七组探讨TA-Sa收缩肌条的作用机制。结果:TASa能增强肌条收缩的振幅、持续时间和收缩面积,并有量效依赖性(振幅:r=0.96,t=23.72,P<0.01;持续时间:r=0.95,t=32.55,P<0.01;收缩面积:r=0.98,t=23.68,P<0.01),但对其收缩频率(NS:3.58±0.15,TASa:3.72±0.21,P>0.05)无影响;异搏定能明显抑制TASa收缩肌条的作用,而阿托品、酚妥拉明、苯海拉明和消炎痛无影响。结论:TASa可能是通过激活Ca2+通道,升高胞浆内Ca2+而加强肌条的收缩。
Objective： To study the contractive effects of the total alkali sophora alopecuroids L （TASa） on sinus ventriculi circular muscle in vitro of rats and the possible mechanisms. Methods ： Adopted constant temperature perfusion of the bath ma- chine, record amplitude, duration, contraction area, frequency of the contraction sinus ventrieuli circular muscle in vitro, TA- Sa divided into five doses groups （ 5, 10, 20, 40, 80mg·L-1 ）, giving different doses of TASa to sinus ventrieuli circular mus- cle in vitro and observed the dosc-effect relationship between muscle contraction and different doses of TASa. Sinus ventriculi cir- cular muscle were divided into seven groups, including control group, TASa group, TASa＋10^-6 mg/L atropine group, TASa＋ 10^-6 mg/L phentolamine group, TASa＋ 10^-6 mg/L benzhydramine group, TASa＋ 10^-6 mg/L antifani group, TASa＋ 10^-7 mg/L isop- tin group. The contraction effects of TASa were investigated. Results： TASa could increase contraction amplitude, contraction duration, contraction area, and has an obvious concentration-dependent contraction of sinus ventriculi circular muscle （ contrac- tion amplitude： r=0.96, t=23.72, P〈0.01; contraction duration： r=0.95, t=32.55, P〈0.01; contraction area： r= 0. 98, t=23. 68, P〈0. 01 ）, but had no effect on contraction frequency （control group： 3.58±0. 15, TASa： 3.72±0. 21, P〉 0. 05 ）. Isoptin could inhibit the circular muscle contraction of TASa, atropine, phentolamine, benzhydramine and antifani had no contraction effect. Conclusion： TASa may activate calcium channel, increase calcium flow to the interior of the cell and in- crease the contraction of sinus ventrieuli circular muscle.
出处
《四川中医》
2012年第11期55-58,共4页
Journal of Sichuan of Traditional Chinese Medicine
基金
四川省教育厅重点项目(08ZA109)
关键词
苦豆子总碱
胃窦
离体肌条
钙离子
Total alkali sophora alopeeuroids L Sinus ventriculi Circular muscle in vitro
