摘要
建立了高效液相色谱法同时测定植物油中4种甾醇烯的方法。样品用石油醚提取,硅胶柱净化,反相C30柱(250 mm×4.6 mm i.d,5μm)分离,乙腈-叔丁基甲醚(75∶25,V/V)作流动相,等度洗脱,紫外检测(235 nm)。结果表明:4种甾醇烯的质量浓度在0.025～1.0 mg/L范围内;峰面积与质量浓度呈良好的线性关系,线性相关系数r2>0.999。在加标水平为0.050,0.10和0.50 mg/kg时,4种甾醇烯的加标平均回收率为89.2%～109.8%;相对标准偏差RSD<8%;方法检出限(LOD)为0.010 mg/kg。本方法已应用于实际样品分析。
A method for the simultaneous determination of four steradienes in vegetable oils by high performance liquid chromatography（HPLC） was established.The sample was dissolved in petroleum ether and passed through a silica gel column,and the analytes were eluted with petroleum ether.The chromatographic separation was carried out on a C30 column（250 mm×4.6 mm i.d,5 μm） using UV detection at 235 nm.Acetonitrile/tert-butyl methyl ether（75 ∶ 25,V/V） was served as mobile phase in isocratic elution.Results showed good linear ranges for 4 steradienes from 0.025 mg/L to 1.0 mg/L with correlation coefficients（r2）0.999.The mean recoveries were in the range of 89.2%-109.8% at three spiked levels of 0.050,0.10 and 0.50 mg/kg,and the relative standard deviations（RSDs） were less than 8 %.The limits of detection（LODs） were of 0.010 mg/kg.The proposed method had been applied in real sample analysis.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第12期1902-1906,共5页
Chinese Journal of Analytical Chemistry
基金
国家质检总局科研项目(No.2012IK184)
上海市教育委员会重点学科建设项目(No.J50704)资助
关键词
高效液相色谱
甾醇烯
植物油
High performance liquid chromatography
Steradienes
Vegetable oils
