摘要
没食子酸衍生物是茶树酚酸中的主要成分,是茶汤中重要的鲜味增强剂,具有抗过敏、抗抑郁和增强认知能力等作用。利用固相萃取(solid-phase extraction,SPE)结合制备高效液相色谱(preparative highperformance liquid chromatography,PHPLC),从茶树鲜叶中分离制备出高纯度的3-O-没食子酰奎尼酸和β-D-没食子酰葡萄糖,并利用LC/TOF-ESI-MS、1H-NMR和13C-NMR对它们的结构进行了鉴定。该方法操作简单、纯化效果好。
Gallic acid derivatives are the chief compounds of phenolic acid in tea.They are important umami-enhancing compounds in tea beverage,and possess the effects of inhibiting IgE producton,enhancing cognition-anting depressant.A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves.The obtained fractions were successfully identified by LC/TOF-ESI-MS,1H-NMR and 13C-NMR.This method is simple,effective for the preparation of gallic acid derivatives.
出处
《茶叶科学》
CAS
CSCD
北大核心
2012年第6期494-499,共6页
Journal of Tea Science
基金
国家自然科学基金(No.30972401
No.31170647和No.31170282)
安徽省自然科学基金(11040606M73)
