摘要
目的 :观察EB病毒潜伏膜蛋白 1(LMP1)表达对鼻咽癌细胞系增殖和细胞周期分布的作用。方法 :用免疫组化 (LSAB)法检测LMP1蛋白的表达 ;用MTT法测定鼻咽癌细胞系的增殖能力 ;用流式细胞术检测细胞周期的分布。结果 :表达LMP1的鼻咽癌细胞系L -CNE1的OD值明显高于LMP1阴性的鼻咽癌细胞系V -CNE1和CNE1(P <0 .0 0 1)。L -CNE1细胞系的G1期细胞百分率明显减少和S期细胞百分率明显增加 ,与V -CNE1和CNE1细胞系比较均有非常显著性差异 (P <0 .0 0 1)。而V -CNE1与CNE1细胞系之间各期细胞百分率则无明显差异 (P >0 .0 5 )。结论 :EBV -LMP1表达可使鼻咽癌细胞系细胞周期分布改变和增殖能力增强。
AIM:To investigate the effect of Epstain-Barr virus latent membrane protein 1 (EBV-LMP1) on proliferation and cell cycle of nasopharyngeal carcinoma (NPC) cells. METHODS:The expression of EBV-LMP1 was detected by immunohistochemical method (LSAB). Proliferation of NPC cells was identified by MTT method. Cell cycle percentage was detected by flow cytometry analysis. RESULTS: OD value of EBV-LMP1 expressive NPC cells L-CEN1 was much higher than that of both EBV-LMP1 negative NPC cells V-CEN1 and CNE1 ( P <0.01). Compared with the cell cycle percentage in both V-CNE1 and CNE1, the percentage of G 1 was significantly decreased and the percentage of S was much increased in L-CNE1 ( P <0.01). But no obvious differences were observed in all cell cycle percentage between V-CNE1 and CNE1 ( P >0.05).CONCLUSION:The expression of EBV-LMP1 on NPC cell might cause some change of cell cycle and enhance cell proliferation.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第6期557-559,共3页
Chinese Journal of Pathophysiology
基金
广东省高教厅重点科研基金资助 (高 95 0 2 )
关键词
鼻咽肿瘤
细胞周期
EB病毒
LMP1
细胞增殖
Epstein-Barr virus
Nasopharyngeal neoplasms
Cell line
Cell division
Cell cycle
