摘要
目的 :探讨细胞凋亡和细胞坏死在急性重症胆管炎中的发生情况与作用。 方法 :向大鼠的胆总管注入大肠杆菌后结扎胆总管 ,制作急性重症胆管炎模型 ,用光镜和TUNEL法观察该模型病理改变与细胞凋亡及细胞坏死的关系。 结果 :随着造模时间的延长 ,模型组的死亡率明显增高 ;肝细胞死亡以凋亡和坏死两种形式发生 ,且凋亡先于坏死发生 ,随坏死的加重而加重 ,大量凋亡细胞出现在坏死灶周边。 结论 :急性重症胆管炎时肝脏除发生细胞坏死外 ,尚有细胞凋亡的发生 ,并且可能起着重要的病理作用。
Objective:To observe the aopotosis and necrosis involved in the acute cholangitis of severe type(ACST).Methods:The ACST animal model was carried out by injecting the Escherichia coli into common bile duct which was then ligated.Using microscopy and TUNEL (TdT mediated dUTP Nick End Labeling),the relationship between apoptosis and necrosis of the hepatocyte in this model was investigated.Results:The death rate in the model group was significantly increased along with the time after the model was done.The death of hepatocyte occurred by two forms of apoptosis and necrosis.The apoptosis was earlier than the necrosis and was gradually serious along with the necrosis.A large amount of apoptosis cells was observed around the necrosis focus.Conclusion:In ACST,the apoptosis except of the necrosis can be arisen,which maybe have the important pathological effect.
出处
《中国中西医结合外科杂志》
CAS
2000年第3期155-157,共3页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
