摘要
目的探讨染料木素抗大鼠动脉粥样硬化的作用。方法采用喂饲高脂饲料及维生素D3联合造模的方法,构建大鼠动脉粥样硬化模型,将40只造模大鼠随机分为模型组、阳性组(辛伐他汀10.0 mg/kg)、染料木素小剂量组(4.5 mg/kg)、染料木素中剂量组(9.0 mg/kg)、染料木素大剂量组(18.0 mg/kg)5组,每组8只;未造模的8只大鼠为正常组。给药组按药物计量分别灌胃,正常组及模型组灌胃等量0.2%羧甲基纤维素钠;每日1次,连续给药14 d,观察染料木素的药理作用。结果①与正常组比较,模型组大鼠心率明显减慢(P<0.05);与模型组比较,染料木素小、中、大剂量灌胃给药14 d后对动脉粥样硬化大鼠心率、血压、呼吸均无明显改变(P>0.05)。②模型组血清三酰甘油、胆固醇、载脂蛋白B、低密度脂蛋白明显升高(P<0.05或P<0.01),染料木素小、中、大剂量给药14 d后能明显降低血清中三酰甘油、胆固醇、低密度脂蛋白和载脂蛋白B含量(P<0.05或P<0.01);模型组血清载脂蛋白A明显降低(P<0.05或P<0.01),染料木素小、中、大剂量给药14 d后血清中的载脂蛋白A明显升高(P<0.05或P<0.01)。③模型组血清总超氧化物歧化酶和一氧化氮合酶明显升高(P<0.05或P<0.01),染料木素小、中、大剂量给药14 d后能明显降低血清中总超氧化物歧化酶和一氧化氮合酶含量(P<0.05或P<0.01)。④镜下观察可见正常组动物主动脉内膜、中膜及外膜完整,内皮下无脂质沉积或斑块形成;模型组主动脉内膜明显增厚、凸凹不平,可见到大量泡沫细胞,严重者细胞崩解,内弹力板断裂,显示了典型动脉粥样硬化的病变;而染料木素小、中、大剂量组和阳性组亦有动脉内膜斑块形成,内膜增厚程度及结缔组织成分均较模型组轻,有散在的泡沫细胞存在。结论染料木素对动脉粥样硬化有一定的治疗作用。
Objective To investigate the effects of genistein （GeM） on atherosclerosis rats. Methods Fed with high fat diet and vitamin D3 to create a rat atherosclerosis model. Successful modeling 40 rats were randomly divided into 5 groups： model group, simvastatin positive control group （10. 0 mg/kg）, genistein low-dose group （4.5 mg/kg）, genistein medium dose group （9 mg/kg）, and genistein high-dose group （18 mg/kg）. Each group had 8 rats. The normal control group and model control group were given the same amount of 0.2% sodium carboxymethyl cellu- lose, twice daily, continuous administration for 14 d to, observe pharmacological effects of genistein. Results ①Compared with the normal group, the heart rate of rats was significantly decreased （P〈0.05）, while GeM with small, medium and large dose had no effect on heart rate, blood pressure,and respiration system after 14 d＇ administration compared with the model group （P〉 0. 05）. ②The serum triglyceride, cholesterol, apolipoprotein B, and low-density lipoprotein levels in of model group significantly increased （P〈0.05 or P〈0.01 ） compared with the normal group. While after administrated with GeM for 14 d, GeM with small, medium and large dose were signifi- cantly reduced in serum triglyceride,cholesterol, low density lipoprotein and apolipoprotein B levels （ P〈0. 05 or P〈0.01 ）. The serum apolipoprotein A level in model group was significantly lower than that in normal group （ P〈0. 05 or P〈0. 01 ）. Three GeM groups after administration with GeM for 14 d, the apolipoprotein A level in serum was significantly higher than that in model group （P〈0. 05 or P〈0. 01 ）. ③The levels of total superoxide dismutase and nitrogen oxide synthase in the serum of model group were significantly increased （ P〈0.05 or P〈0.01 ） , while those of in Gen groups significantly decreased （P〈0. 05 or P〈0. 01 ）. ④Pathology results showed that normal rats＇ aortic intima, the mid- membrane, outer membrane were integrity and there were no lipid deposition or plaque under the en- dothelium, while the aortic intima in models was thickening and uneven. A large number of foam cells, severe by cell collapse, internal elastic lamina fracture were seen. That clearly suggested that the typical atherosclerotic lesions had formed. Arterial plaques, degree of intimal thickening and connective tissue components with scattered foam cells existed and these changes were much lighter than the model group. Conclusion Gen has a therapeutic effect on atherosclerosis.
出处
《转化医学杂志》
2012年第3期139-143,共5页
Translational Medicine Journal
