摘要
目的分析血液感染患者的病原菌分布特点及耐药特性,为临床合理使用抗菌药物提供依据。方法收集临床血培养阳性患者560例,菌株用VITEK 2 compact鉴定到种,药敏试验采用纸片扩散法(K-B法),结果按临床实验室标准化协会(CLSI)2010年版标准判读,用WHONET 5.6软件进行耐药性分析。结果 560例患者共分离出568株菌,其中革兰阴性菌占68.0%(386/568),革兰阳性球菌占29.0%(165/568),真菌占3.0%(17/568)。大肠埃希菌分离率最高为34.9%(198/568);其次为肺炎克雷伯菌为16.9%(96/568);葡萄球菌中以凝固酶阴性葡萄球菌检出率最高为15.5%(88/568);金黄色葡萄球菌为9.0%(51/568)。大肠埃希菌和肺炎克雷伯菌对碳青霉烯类抗菌药物100%敏感,对其他抗菌药物有不同程度的耐药;未发现对万古霉素耐药葡萄球菌。结论血液感染菌株以革兰阴性菌为主,大肠埃希菌是主要感染细菌,凝固酶阴性葡萄球菌的感染不容忽视。
Objective To analyze the pathogenic bacteria distribution and drug resistance of blood infection. Methods A total of 560 cases with positive blood cuhure results were included in the study. Species of the bacteria were identified with VITEK 2 compact. Antimicrobial susceptibility testing was conducted with disk diffusion （KB method）. Testing results were interpreted using 2010 CLSI Standards for Antimicrobial Susceptibility Testing. Drug resistance was analyzed using WHONET 5.6 software. Results The isolated 568 strains consisted of Gram - negative bacilli （386/568, 68.0% ）, Gram - positive bacilli （165/568, 29.0% ）, and fungi （ 17/568, 3.0% ） . Escherichia coli had the highest isolation rate of 34. 9% （ 198/568）, followed by Klebsiella pneumoniae 16. 9% （96/568）. Coagulase negative staphylococcus had the highest isolation rate of 15.5 % （88/568） among Staphylococcus, followed by Staphylococcus aureus 9.0% （51/568）. Escherichia coli and Klebsiella pneumoniae were 100% sensitive to carbopenems, while exhibited varied resistance to other drugs. Staphylococcus was 100% sensi- tive to vancomycin. Conclusion The bacteria causing blood infection are mainly Gram - negative bacilli. Escherichia coli are the major pathogenic bacteria. The infection of Coagulase negative staphylococcus should be treated with caution.
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第34期4016-4018,共3页
Chinese General Practice
关键词
血培养
菌群分布
耐药性
Blood cultured
Distribution of bacteria
Drug resistance
