摘要
目的:比较研究Aβ免疫阳性产物在老年APP/PSl双转基因(2xTg)与APP/PSl/Tau三转基因(3xTg)阿尔茨海默病模型小鼠海马的分布。方法:采用15月龄2xTg与同龄3xTg,分别进行Aβ单克隆抗体6E10免疫组化检测Aβ阳性神经元及斑块。结果:在海马区2xTg组Aβ沉积多发生于细胞外,形成大量Aβ阳性斑,而3xTg组则主要沉积于细胞内。结论:这种Aβ分布的差异可能与3xTg模型早期即有神经元丢失有关。
Objective： To compare the distribution ofAI3 immunoreactive in the hippocampus of the elder APP / PSI double trans-genic mice （2xTg） and APP / PSI / Tau （3xTg） triple transgenic mice model of Alzheimer＇s disease. Methods： 15-months-old 2xTg and the age-matched mouse of 3xTg were used in this experiment. 6E10 Immunohistochemical staining was used to show the distribution of Aβ immunoreactive in the hippocampus. Results： In the hippocampus, the deposition of Aβ in 2xTg usually occurs in the extracellu-lar and form a lot of positive plaques while in the 3xTg it occurs intracellularly. Conclusion： The difference in the distribution orAβ is likely to be related to the neuronal loss which existed in the 3xTz early.
出处
《现代生物医学进展》
CAS
2012年第32期6253-6255,6245,共4页
Progress in Modern Biomedicine
