摘要
目的:探讨生长抑素受体(SSTR)1～3亚型蛋白在大肠癌组织中表达及其与临床病理学的关系。方法:收集41例大肠癌患者手术切除肿瘤组织标本及23例远癌组织(指距肿瘤活检切除边缘3～6 cm的组织)标本,采用免疫组化PV-9000二步法检测其SSTR1～3亚型蛋白的表达;采用Fisher确切概率法进行统计学分析,并分析与临床相关因素的关系。结果:41例大肠癌组织中SSTR1～3亚型蛋白的阳性表达率分别为65.8%(27/41)、61.0%(25/41)和56.1%(23/41),均较远癌组织表达率低(P<0.05)。SSTR1～3亚型蛋白在大肠癌组织中的表达与患者的性别、有无淋巴结转移和临床分期无关,SSTR2和SSTR3亚型蛋白与肿瘤的分化程度及肿瘤的部位无关;而SSTR1亚型蛋白在高/高中分化的大肠癌组织中的表达高于低中分化和低分化大肠癌组织中的表达,且直肠癌中的表达较结肠癌低(P<0.05)。结论:SSTR1亚型蛋白是大肠癌组织中的主要表达形式,可能与大肠癌的发生发展有关。
Objective： To investigate the SSTR subtypes expression and distribution in tissues of colorectal carcinoma and relationship with their clinicopathological feature. Methods： The total 41 cases of colorectal carcinoma tissues and their adjacent tissues （23 cases） were involved in this study at random. The expression of SSTR1-3 subtype proteins in 41 cases of the colorectal carcinoma tissues were detected by immunohistochemistry used by two-step way. The expressions of SSTR1-3 subtype proteins and clinical pathology were analyzed with Fisher＇s exact probabilities method and Spearman＇s non-parametric analysis. Results： The expression levels of SSTR1-3 subtype proteins in the colorectal carcinoma tissues were 65.8% （27/41） ,61.0% （25/41） and 56. 1% （23/41） respectively, which showed that the expressions of SSTR1-3 subtype proteins were significantly lower than that in adjacent normal colorectal carcinoma tissues （P 〈 0. 05 ）. It also showed that the expressions of SSTR1-3 subtype proteins were not correlated with gender, TNM staging and metastasis, and the expression of SSTR1 in high differentiated tissues was significantly higher than poorly differentiated tissues. Conclusion： SSTR1 subtype protein is the main expression pattern in colorectal carcinoma and it may be closely related with development and genesis of colorectal carcinoma.
出处
《现代医学》
2012年第6期645-648,共4页
Modern Medical Journal
关键词
大肠癌
生长抑素
生长抑素受体
免疫组织化学
colorectal carcinoma
somatostatin
somatostatin receptor
immunohistochemistry
