摘要
目的通过研究丹酚酸A(Salvianolic acid A,SalA)对人肺癌A549细胞PTEN_PI3K/AKT信号通路的影响,探讨SalA抑制肺癌细胞增殖的作用机制。方法以人肺癌A549细胞为靶细胞,运用高内涵细胞成像分析系统,检测SalA对A549细胞增殖的影响;MTT法观察SalA对A549细胞增殖影响的量效关系;通过Western Blotting实验,检测SalA用于人肺癌A549细胞,对PTEN蛋白、磷酸化AKT(P-AKT)蛋白、非磷酸化AKT(T-AKT)蛋白的影响。结果 SalA对A549细胞增殖有抑制作用,并呈现剂量依赖性,而SalA可以促进PTEN蛋白及T-AKT蛋白的表达,抑制P-AKT蛋白的表达。结论 SalA具有抑制肺癌A549细胞增殖的作用,人肺癌A549细胞中存在有活性的PTEN_PI3K/AKT信号通路,SalA可能通过抑制PTEN_PI3K/AKT信号通路中P-AKT蛋白的表达,促进PTEN蛋白及T-AKT蛋白的表达,来抑制肺癌细胞增殖,促进癌细胞凋亡。
OBJECTIVE To study the effect of Salvianolic acid A（SalA） on PTEN_PI3K/AKT signaling pathway of human lung cancer cell line A549,to explore the mechanism of SalA in vitro inhibition of lung cancer cell.METHODS Human lung cancer cell line A549 were used as target cells,and High Content Imaging Analysis System was used for detecting the effect of SalA on inhibits growth of A549 cells.MTT method was used to observe the effects of different concentrations of SalA on A549 cells.Protein levels of PTEN、P-AKT and T-AKT were measured by Western-blotting methods.RESULTS The growth of A549 cells was inhibited by SalA,and show a certain degree of dose dependent.Results from Western Blotting showed that SalA up-regulated PTEN protein level and T-AKT protein level,as well as down-regulated P-AKT protein level.CONCLUSION SalA can inhibit A549 cells.We could fund active PTEN_PI3K/AKT signaling pathway from Human lung cancer A549 cells.These results indicate that Sal A negatively mediates lung cancer cell line A549 growth or apoptosis most likely through positively regulating PTEN,T-AKT and P-AKT protein level by PTEN_PI3K/AKT signaling pathway.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2012年第6期541-543,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省高校优势学科建设工程项目(PAPD)
国家自然科学基金(81273638)
