摘要
以正常培养条件下的莱茵衣藻为对照,通过数字表达谱技术对缺氮诱导3 d的藻细胞进行转录水平上的检测,并结合pathway分析。结果表明:差异表达基因共有482个,其中上调表达基因108个,下调表达基因374个;这些差异表达的基因共分布在85个pathways中。此结果为进一步揭示氮元素缺乏诱导微藻油脂积累的分子机理提供有用信息。
Digital gene expression profiles were performed on Chlamydomonas reinhardtii cells grown in HSM and HSM-N media for 3 days, and the cells grown in normal HSM medium served as control. Total 482 differentially expressed genes were identified after nitrogen starvation, among of which, 108 genes were up-regulated and 374 genes were down-regulated by N starvation. These genes involved in 85 pathways. These results provided information for deeply revealing the molecular basis underlying nitrogen deficiency-induced triacylglycerols accumulation.
出处
《热带农业科学》
2012年第11期66-70,共5页
Chinese Journal of Tropical Agriculture
基金
中央级公益性科研院所基本科研业务费专项(No.ITBB110507)
海南省工程技术研究中心专项:热带能源微藻新藻种培育及产业化研究(No.GCZX2011006)
利用紫外诱变技术选育优良微藻藻种及培养条件优化(No.GCZX2012004)
关键词
莱茵衣藻
数字表达谱
氮胁迫
Chlamydomonas reinhardtii
digital gene expression
nitrogen starvation
