摘要
从双孢菇基质中诱集得到的一种隐杆属线虫(Caenorhabdtis sp.CNM)具有4天快速致死大蜡螟(Galleria mellonella),为探讨其生防潜力,对其进行了侵染力、抗逆能力及运动能力的研究。结果显示:小杆线虫CNM对大蜡螟的LD50(48 h)为46.6 IJs.larva-1,LC50(48 h)为53.8 IJs.cm-2。CNM线虫在42℃,40℃和38℃水浴下全部个体死亡所需时间分别为45 min,20 h和55 h。CNM线虫在RH 50.5%、温度25℃条件下存放12 h,虽有46.0%个体存活,但侵染力下降43.3%。有51.7%的CNM线虫在30℃环境下经过24 h可爬行3.6 cm寻找大蜡螟寄主体液。由此认为,CNM线虫可以作为有效的生物防治制剂,用于害虫的防治。
One species of insect nematode （Caenorhabditis sp. CNM）, which can cause Galleria mellonella death within 4 days, was discovered in the compost of Agaricus bisporus through the Galleria bait method. In order to dis- cover the biological-control potential of CNM in the field application, we investigated its biology characteristics, es- pecially the infectivity and anti-stress resistance. The result of its infectivity to G. mellonella showed that the larger of the dosage, the better of its infectivity. LD50 of CNM to G. mellonella was 46. 6 IJs per larva, and the LC50 is 53.8 IJs.cm-2 at48 h. After 45 min, 20 h and 55 h, all of the nematodes died with water bathing of 42℃, 40℃ and 38℃. There＇re still 46.0% of the nematodes alive after 12 h under 25℃, 50. 5 % RH, and the infectivity decrease was 43.3%. Besides, there were re 57. 1% of nematode that can move for as far as 3.6 cm under 30℃. So Cae- norhabditis sp. CNM strain has the bio-control potential with good infectivity, heat resistance and desiccation.
出处
《浙江农业学报》
CSCD
北大核心
2012年第6期1058-1063,共6页
Acta Agriculturae Zhejiangensis
基金
现代农业产业技术体系(CARS-29)
农业公益性行业科研专项(201003043-02)
浙江省温州市科技局项目(2009N12)
关键词
双孢蘑菇
隐杆线虫
生物防治
侵染力
抗逆能力
Agaricus bisporus
Caenorhabditis
biocontrol
infectivity
anti-stress resistance
