摘要
大气颗粒物PM2.5受到人们广泛关注,利用模式生物对PM2.5健康风险进行系统环境医学角度和生物监测实践的研究尚少。本文以秀丽隐杆线虫(Caenorhabditis elegans)为材料,于2012年4月初在长春市典型生活区采集PM2.5进行毒理学试验。结果表明,热休克蛋白基因hsp70缺失型突变体线虫受PM2.5影响较大,头摆、咽动和繁殖率等指标与胁迫强度存在较好的线性关系,PM2.5的毒性效应具有遗传特征,可影响子代幼体的发育。hsp70在机体对PM2.5的胁迫响应中作用明显,秀丽隐杆线虫突变体可作为PM2.5的指示生物,对PM2.5进行有效的快速风险评价。通过模式生物应激响应,发现PM2.5可能对人体行为、免疫以及生殖等方面都有不同程度的影响,本研究对大气污染区的环境风险预警与人体健康评价研究提供了理论视角和实践可能。
Atmospheric particulate matter PM2.5 is extensively concerned by people recently. Studies with model organisms on health risk of PM2.5, the system environment medical practice and biological monitoring are still lack. In this paper, Caenorhabditis elegans was used as a model organism to study the stress response of PM2.5, taking from a typical living area of the Changchun at the beginning of April 2012. The result showed that （hsp70 knocking down mutant nematodes） were very sensitive to PM2.5, their head thrash, the pharyngeal pump and reproduction rates had good linear relationships with the exposure of PM2.5. The toxicity effect of PM2.5 had genetic characteristics, as PM2,5 might lead the filial generation larval development arrest. Heat shock protein gene hsp70 had played an important role in the PM2.5 stress response effect. C. elegans mutants could be used as a biological indicator for fast and effective risk assessment of PM2.5. Through stress response of the model biological study, we found that PM2.5 might affect human behavior. Our study provided a theoretical perspective and practice for the environment and human body health research on an atmospheric pollution area.
出处
《城市环境与城市生态》
CAS
CSCD
2012年第6期10-13,共4页
Urban Environment & Urban Ecology
基金
国家自然科学基金(41001339
41271485
40901036)
吉林省科技发展计划项目(201201004)
