摘要
笃斯越橘（Vaccinium uliginosum）又称蓝浆果,属杜鹃花科（Ericaceae）越橘属（Vaccinium）（李亚东,2001）,广泛分布于北半球。中国野生笃斯越橘资源主要分布在大兴安岭、小兴安岭地区（李亚东等,2001）。笃斯越橘果实具有营养保健作用（李亚东等,2004）,包括消除眼睛疲劳、软化血管、
In order to screen culture conditions of a bioreactor for multiplying blueberry（Vaccinium uliginosum）,the raft culture method was used in an air-lift bioreactor to study effects of inoculation density,air volume,and light intensity on proliferation and growth of shoots.The results indicated that a mass of shoots were able to be produced by using raft culture in a bioreactor.Inoculation density affected multiplication of blueberry,60 inoculates in a 5 L bioreactor were the most suitable inoculation density,which led 38.6 adventitious shoots per explant,and total 2 316 vigorous shoots per bioreactor were obtained.Air volume rate of 100 mL·min-1 had the best effect on proliferation,and it produced significantly higher growth of shoots,more shoot numbers,greater fresh and dry mass than did the air volume groups of 50 and 150 mL·min-1.Multiplication of shoots was also affected by light intensity,and 45 μmol·m-2s-1 was better than either 30 or 60 μmol·m-2s-1 for producing dark green and vigorous shoots in the bioreactor.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第11期130-133,共4页
Scientia Silvae Sinicae
基金
国家自然科学基金项目"生物反应器内铁皮石斛圆球茎生长动力学及有效物质积累规律的研究"(31160079)
关键词
笃斯越橘
组织培养
生物反应器
接种量
通气量
光强
blueberry（Vaccinium uliginosum）
tissue culture
bioreactor
inoculation density
air volume
light intensity
