摘要
目的研究严重少精子症和无精子症与Y染色体无精子因子(AZF)微缺失之间的关系。方法采用多重聚合酶链反应技术对103例原发无精子症、72例原发严重少子精症患者及60例正常生育男性进行AZFa、AZFb、AZFc 3个区域微缺失分析。结果 60例正常生育男性未发现Y染色体AZF区域微缺失,175例生精障碍患者中发现AZF微缺失19例,总缺失率为10.9%。其中11例无精子症患者和4例少精子症患者的缺失发生在AZFc区域,缺失率为8.6%;1例无精子症患者和2例少精子症患者发生AZFb、AZFc双重缺失,缺失率为1.7%;1例无精子症患者发生AZFa、b、c 3个区域同时微缺失,缺失率0.6%。生精障碍组与正常生育男性组比较Y染色体AZF区域微缺失率差异具有显著性(P<0.01)。结论 Y染色体AZF区域微缺失是引起男性无子精症、少精子症的重要原因之一。采用多重聚合酶链反应技术对原发无精子症、少精子症患者在单精子注射(ICSI)之前进行微缺失筛查是必要的。
Objective： To investigate the relationship between microdeletion of azoospermia factor（AZF）and male infertility.Methods： Multiplex PCR was used to detect Y chromosome microdeletion in AZFa,AZFb and AZFc on 103 cases of idiopathic azoospermia,72 cases of severe idiopathic oligozoospermia,and 60 cases of healthy male controls.Results： No microdeletion was found in 60 controls.Y chromosome microdeletion was found in 19 of 175 azoospermia patients,the total p revalence rates of microdeletion was 10.9%.There were 15 cases（11 for azoospermia,4 for severe oligozoospermia）in AZFc（8.6%）;3 case（1 for azoospermia,2 for severe oligozoo spermia） in AZFb＋c（1.7%）;1 case（1 for azoospermia） in AZFa＋b＋c（0.6%）.According to statistics,the difference between two groups was significant（P0.001）.Conclusion： Y chromosome microdeltions is an important reason of azoospermia,screening of Y chromosome microdeletions for azoospermia patients before ICSI treatment is essential.
出处
《中国优生与遗传杂志》
2012年第12期25-26,21,共3页
Chinese Journal of Birth Health & Heredity
基金
广东省科技计划项目
编号:2010B031600081
