摘要
目的探讨促红细胞生成素(EPO)对糖尿病大鼠心肌纤维化及心功能的影响。方法采用链脲佐菌素一次性腹腔注射成功建立糖尿病动物模型雄性SD大鼠20只,随机均分为模型对照(A)组和EPO处理(B)组;另取10只SD大鼠作为空白对照(C)组。12周后,称取心脏重量和大鼠体重,计算心体比;Western blot检测心肌组织转化生长因子β(TGF-β)表达;天狼猩红染色测心肌组织胶原含量;心脏超声检测心功能。结果与C组比较,A组心肌组织TGF-β蛋白表达和胶原含量明显增加(P<0.01),心体比、左室舒张末期内径(LVDd)和左室收缩末期内径(LVDs)明显增加(P<0.05),射血分数(EF)明显下降(P<0.01)。与A组比较,B组心肌组织TGF-β蛋白表达及胶原含量均明显减少(P<0.01),EF值增加(P<0.05),LVD d和LVDs明显减少(P<0.05)。结论EPO通过减少糖尿病大鼠TGF-β的表达来逆转心肌间质纤维化,改善心功能。
Objective To investigate the effects of erythropoietin on myocardial interstitial fibrosis and cardiac function in diabetic rats.Methods After successfully established the STZ-induced rat diabetic models,20 male SD rats were equally randomized into two groups of A（model control） and B（treated with EPO）.Another 10 SD rats were taken as blank control（group C）.Twelve weeks later,echocardiography was conducted for evaluating cardiac function.The ratio of heart weight to body weight was calculated.The expression of transforming growth factor-β（TGF-β） in myocardium was detected by Western blot.Myocardial collagen content was measured by Picrosirius Red staining.Results Compared to group C,the expression of TGF-β in myocardium and myocardial collagen content were increased（P0.01）,the ratio of heart weight to body weight and systolic-and diastolic internal diameters were all increased,but ejection fraction of the left ventricule was reduced（P0.05）.Compared to group A,the expression of TGF-β in myocardium and myocardial collagen content were significantly lower and cardiac function was much better in group B（P0.05）.Conclusion EPO may reduce cardiac interstitial fibrosis and improve cardiac function in diabetic rats by decreasing TGF-β expression in myocardium.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第23期2796-2798,共3页
Jiangsu Medical Journal
关键词
糖尿病
促红细胞生成素
心肌纤维化
转化生长因子Β
Diabetes mellitus
Erythropoietin
Myocardial interstitial fibrosis
Transforming growth factor beta
