摘要
目的:研究Smad7及上皮间质转化(EMT)相关标志在胆管癌组织中的表达及与临床病理因素的关系。方法:采用免疫组化检测41例胆管癌和癌旁组织中Smad7、E-cadherin和Vimentin的表达,同时结合临床病理资料及临床预后进行分析。结果:41例胆管癌组织中Smad7、Vimentin的阳性表达率〔68.3%(28/41)、70.7%(29/41)〕及E-cadherin缺失率〔51.2%(21/41)〕明显高于相应癌旁组织〔30.0%(3/10)、30.0%(3/10)和10.0%(1/10)〕;Smad7、Vimentin和E-cadherin的表达与淋巴结转移、神经脉管浸润差异有统计学意义,P<0.05;而与性别、年龄、肿瘤部位、肿瘤类型和肿瘤分化程度等无关,P>0.05。胆管癌组织中E-cadherin的缺失及Vimentin的阳性表达与Smad7表达有相关性,P值分别为0.013和0.018;Smad7高表达者往往术后无病生存期及总生存期均较短,P值分别为0.044、0.034。结论:胆管癌在其癌变与浸润转移过程中可能发生了EMT现象,Smad7蛋白在胆管癌组织中会随进程发展而增加,可能与Smad信号通路激活,机体反馈性调节增加有关,且其有望做为一种预后指标应用于临床评估。
OBJECTIVE： To investigate the expression of Smad7 and epithelial-mesenchymal transition related markers in cholangiocarcinoma tissues arid its relation with clinicopathological factors. METHODS： The expression of Smad7, E-cadherin,vimentin protein in 41 cases of cholangiocarcinoma and the adjacent tissues was detected by immunohistochemistry. The relationship of the expression with clinicopathological characteristics and prognosis of cholangiocarcinoma was analyzed. RESULTS： The positive expressions of Smad7 and Vimentin [68.3 % （21/41）, 70.7 % （29/41）] in cholangiocarcinoma tissue were dramatically higher than those in the adjacent tissues （30.0 % （3/10）. 30.0 % （3/10）]. However, Rate of loss of E-cadherin was contrary [51.2 % （21/41）, 10.0 % （1/10）]. Smad7 and Vimentin, E-cadherin expressions were significantly higher in tumors with lymph node me tastas and perineural invasion （P〈0.05）. There were no correlation with other factors such as gender, age, tumor location, tumor type,tumor differentiation degree （P〉0.05）. The Protein levels of Smad7, E cadherin and Vimentin in tumor samples showed a significant correlation （P=0. O13;P=0. 018）. Postoperative follow-up of patients with cholangiocarcinoma demonstrated that the survival rate was significantly higher in patients with negative Smad7 expression than those with positive Smad7 expression（P= 0. 044;P= 0. 034）. CONCLUSIONS： EMT phenomenon may occur in the process of CCA invasion and metastasis. Smad7 expression may be increased with development of tumor and it may play a potential role as a prognostic indicator for clinical assessment of CCA.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第21期1651-1655,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
安徽省自然科学基金(1208085mh176)
