摘要
目的对链脲佐菌素(STZ)诱导的C57BL/6和eNOS基因敲除(eNOS-/-)小鼠1型糖尿病模型(T1DM)进行对比研究,探讨两类糖尿病小鼠模型的视网膜功能及视网膜血管的病理变化。方法 6-8周龄的C57BL/6和eNOS-/-小鼠腹腔内注射STZ建立T1DM模型。模型建立前后分别行视网膜电图(ERG)检查、荧光血管造影、免疫荧光染色及视网膜神经节细胞计数。结果C57BL/6和eNOS-/-糖尿病小鼠ERGa、b波振幅降低、神经节细胞减少(P<0.05),eNOS-/-糖尿病小鼠视网膜血管迂曲、纤细。与C57BL/6糖尿病小鼠相比,eNOS-/-糖尿病小鼠的视网膜功能及视网膜血管的病理改变更严重、迅速。结论与C57BL/6T1DM模型相比,eNOS-/-T1DM模型能更全面地反映糖尿病视网膜病变的发生、发展,为研究糖尿病及糖尿病视网膜病变提供了一个更理想的动物模型。
Objective To compare the retinal function and retinal vascular pathologies in C57BL/6 and eNOS-knockout （eNOS-/-） mouse models of type 1 diabetes mellitus （TIDM） induced by streptozotocin （STZ）. Methods TIDM models were established in 6- to 8-week-old C57BL/6 and eNOS-/- mice by intraperitoneal STZ injection. Electroretinogram （ERG） examination, fluorescein angiography （FFA）, immunofluorescence staining and retinal ganglion cell counts were carried out before and after STZ injection. Results Diabetic C57BL/6 and eNOS＋ mice showed significantly lowered a-wave and b-wave amplitude in ERG and reduced number of retinal ganglion cells （P〈0.05）, and the retinal vessels in diabetic eNOS-/- mice became tortuous. Compared with diabetic C57BL/6 mice, diabetic eNOS-/- mice showed more severe pathological changes in retinal function and retinal vessels with also more rapid onset of pathologies. Conclusion Compared with C57BL/6 mouse models, eNOS-/- mouse models of TIDM can better represent the occurrence and development of diabetic retinopathy, thus providing an ideal model for diabetes and diabetic retinopathy studies.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第12期1683-1688,共6页
Journal of Southern Medical University
基金
国家自然科学基金(30973251)~~
