摘要
目的:探讨胱蛋白M(Cystatin M)在乳腺癌及转移癌中的表达和临床意义。方法:采用RT-PCR检测108例乳腺癌标本,30例转移癌标本及24例癌旁正常乳腺组织中Cystatin M mRNA,分析Cystatin M基因表达与临床病理参数和预后的关系。结果:乳腺癌标本中Cystatin M表达水平在Ⅰ/Ⅱ期乳腺癌患者中较高,而在Ⅲ/Ⅳ期乳腺癌患者中较低,差异有统计学意义;Cystatin M在5 cm以下肿瘤中表达较高,而在5 cm以上肿瘤中表达较低,差异有统计学意义;乳腺癌标本中Cystain M表达与正常乳腺组织中CystatinM表达差异无统计学意义;乳腺癌标本中Cystatin M与转移癌标本中Cystatin M表达差异无统计学意义;乳腺癌标本中CystatinM表达水平与乳腺癌患者是否发生淋巴结转移,淋巴结转移数,组织学分级,病理学类型无相关性;乳腺癌标本中Cystatin M表达水平和转移癌标本中Cystatin M表达水平与人表皮生长因子受体2状态(HER-2),雌激素受体状态(ER)及孕激素受体状态(PR)无相关性;乳腺癌标本中Cystatin M表达较高的组患者中位总生存期比Cystatin M表达较低的组患者中位总生存期要长,两者之间差异无统计学意义。结论:Cystatin M为监测乳腺癌发生浸润转移的可靠指标仍需探讨。
Objective： This study aims to explore the expression and clinical significance of cystatin M in breast cancer. Meth-ods： Reverse transcription polymerase chain reaction was used to measure the level of cystatin M mRNA in 108 samples of breast can -cer, 30metastatic cancer, and 24normal breast tissues. The relationship between the expression of cystatin M gene in human breast can-cer and the clinilcopathological features was analyzed. Results： The cystatin M expression levels in breast cancer are correlated with TNM staging and clinical size of the tumor. The expression levels of cystatin M in breast cancer tissue are not significantly different from normal breast tissues and metastatic cancer tissues. Cystatin M expression levels in breast cancer are not correlated with lymph node metastasis, axillary lymph node status, histological grade, or pathological type. Cystatin M expression levels in breast cancer and metastatic cancer are not correlated with Her-2, ER, or PR status. Cystatin M expression levels in breast cancer are correlated with the overall survival. Conclusions： Whether cystatin M can be used as a prognostic marker for breast cancer metastasis still needs further studies.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第22期1792-1795,共4页
Chinese Journal of Clinical Oncology
