摘要
目的 应用双重套式 PCR技术同时检测发酵支原体 (Mf)和穿通支原体 (Mpe)。方法 以支原体 16 Sv RNA基因为靶基因 ,外套引物为 Mf 与 Mpe共用、内套引物则分别为 Mf 种特异和 Mpe种特异 ,建立双重套式扩增体系。结果 本双重套式 PCR不仅能分别扩增 Mf 和 Mpe的特异性 DNA,并能同时扩增 Mf 和 Mpe出现二条特征性条带 ,产物经测序证实。结论 双重套式 PCR是一种灵敏、特异和快速的 Mf 和
Aim In order to detect Mycoplasma fermentans (Mf)and Mycoplasma penetrans (Mpe) at the same time dual nested-PCR(DN-PCR) technique was established Method Mpe the same time PCR Primers were designed according to published 16s rRNA Sequences of Mf and Mpe, the outer primers were univevsal to both Mf and Mpe and the inner was Mf、Mpe specie-specific respectively. Two-step amplification procedures were perfomed. Result Chromo some DNAS of Mf and Mpe gave characteristic DN-PCR prafiles which were differeht from other my coplasmas or microorganism tested. Conclusion The DN-PCR method was established for the detection of Mf and Mpe at the same time which was sensitive specific and rapid.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第4期50-52,共3页
Chinese Journal of Zoonoses
