摘要
本文对凹凸羊膜(CCAM)的生物力学特性和生物学活性做进一步研究,以探讨其作为生物移植材料的优越性。将羊膜上皮面朝下对折、真空压缩制备成CCAM,同时将羊膜切割成细条,随机选取制备好的16条CCAM在电子万能试验机上进行单向拉伸性能测试,分别以单层羊膜和双层羊膜作对照,得出抗拉强度、断裂伸长率、弹性模量等。免疫放射法分析CCAM的细胞因子残存情况。CCAM厚度介于单、双层羊膜之间,复水15min后形态可完全恢复。CCAM的抗拉强度较单、双层羊膜明显增加(P<0.05),断裂伸长率最高(P<0.05),弹性模量小于单、双层羊膜(P<0.05)。放射免疫分析发现CCAM中EGF、FGF、HGF等10种成分明显小于新鲜羊膜含量,差异有统计学意义(P<0.05),CCAM中EGF、FGF、HGF、TGF-β、PDGF、NGF 6种因子含量明显高于单、双层干燥羊膜的因子含量(P<0.05),而IGF、VEGF、BDNF、CNTF 4种因子含量与单、双层羊膜比较差异无统计学意义(P>0.05)。结果表明:CCAM复合材料厚度薄,其柔韧性和可塑性优于单、双层羊膜,细胞因子保存良好,作为生物移植材料具有更大的优越性。
This paper conducted research on biomechanical characteristics and biological activity of concavityconvex amniotic membrane （CCAM） and discussed its superiority as ocular surface repair material. Folding and compression with vacuum of fresh amniotic membrane were used to prepare CCAM. After cutting the striga of CCAM, sixteen CCAM tissue section were chosen at random to test their tensile strength using electronic universal testing machine. The bilayer amniotic membrane （BAM）, the doubledeck amniotic membrane （DAM） and the monolayer amniotic membrane （MAM） were as controls. The test parameters included yield strength, tensile strength, elongation at break, elastic modulus and so on. The cytokines of fresh amniotic membrane （FAM）, MAM and CCAM were ana lyzed by radioimmunoassay method. The CCAM was obviously thicker than MAM and DAM. After 15min in PBS, the CCAM tissue can recover the normal shape. The tensile strength and the elongation at break of CCAM werehigher than those of the MAM and the DAM （P〈0. 05）. The elastic modulus of the CCAM was smaller than that of the MAM and the DAM （P〈0. 05）. The content of 10 cytokines β-epidermal growth factor（EGF）, fibroblast growth factor （FGF）, b-fibroblast growth factor b-FGF, hepatocyte growth factor （HGF）, transforming growth faetor-β （TGF-β）, insulin-like growth factor（IGF）, platelet-derived growth factor （PDGF）, vascular endothelial growth factor（VEGF）, nerve growth factor（NGF）, brain-derived nellrotrophie factor （BDNF）, ciliary neurotrophic factor （CNTF）] of CCAM decreased significantly compared with the FAM and increased significantly compared with MAM and DAM in 6 cytokines （EGF, FGF, HGF, TGF-β, PDGF, NGF（P〈0.05）. The CCAM composites is thinner and has higher cytokine content than MAM, and better biomechanieal properties than the MAM and the DAM, showing the superiority as ocular surface repair material.
出处
《生物医学工程学杂志》
CAS
CSCD
北大核心
2012年第6期1114-1118,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(81160118)
江西省科技支撑计划项目资助(20111BBG70026-2)
江西省自然科学基金资助项目(20114BAB21502)
江西省卫生厅科技计划面上项目资助(20121026)
江西省教育厅青年科学基金资助项目(JJJ12158)
关键词
凹凸羊膜
制备
形态学
生物力学
Concavity-convex amniotie membrane （CCAM） Preparation Morphology Biomechanics
