摘要
目的 :研究氯胺酮和γ 羟基丁酸钠对支气管平滑肌腺苷酸环化酶 (AC)和磷酸二酯酶 (PDE)活力的影响。方法 :离体实验 ,分为对照组、氯胺酮组和γ 羟基丁酸钠组 ,每组 8只家兔。氯胺酮和γ 羟基丁酸钠的实验浓度分别为2 3× 10 -4 M和 3 0× 10 -3 M。结果 :氯胺酮和γ 羟基丁酸钠对AC活力无明显影响 ,但均可显著抑制PDE的活力。两组PDE活力分别为 2 42± 0 6 3U和 3 6 0± 0 87U ,与对照组 4 5 8± 0 42U相比 ,分别P <0 0 1和P <0 0 5。结论 :氯胺酮和γ 羟基丁酸钠均可减少支气管平滑肌中cAMP的降解 ,这可能是氯胺酮和γ 羟基丁酸钠在麻醉中使支气管平滑肌张力下降的原因之一。
Objective:To explore the effects of ketamine and γ hydroxybutyrate sodium(γ OH) on adenyl cyclase(AC) and phosphodiesterase(PDE)of the rabbit bronchial smooth muscle in vitro.Methods:The rabbit’s bronchial smooth muscle samples were divided into control group,ketamine group and γ OH group with 8 samples each.The reactive concentration of ketamine was 2 3×10 -4 M or γ OH was 3 0×10 -4 M.Results:The activity of AC was not affected by ketamine or γ OH, but the activity of PDE could be inhibited by ketamine or γ OH.The activity of PDE was 2 42±0 63U in ketamine group and 3 60±0 87U in γ OH group.Compared with control group 4 58±0 42U,the activity of PDE was obvious lower in ketamine group ( P< 0 01) or in γ OH group ( P< 0 05).Conclusion:Both ketamine and γ OH can slow down the degradation of cAMP.This may be one of the reasons for lowering the tone of the bronchial smooth muscle during anesthesia.
出处
《临床麻醉学杂志》
CAS
CSCD
2000年第5期235-236,共2页
Journal of Clinical Anesthesiology
