摘要
应用苗期群体抗性测定方法,鉴定了华航1号、华航8号、胜巴丝苗等10个水稻品种对稻褐飞虱的抗性;应用高效液相色谱(HPLC)技术对其中6个品种的特征次生物质色谱峰进行了测定,并分析了主要抗性峰值与抗性级别之间的关系。结果表明:Mudgo为高抗,粳籼89、华航8号、胜巴丝苗为抗虫品种;小农占、籼小占、华航1号和粤香占呈中抗反应;双桂呈感虫反应,TN1为高感品种,这一鉴定级别与田间稻褐飞虱种群增长趋势指数变化相一致;稻褐飞虱在TN1上的种群趋势指数达28.079,中抗品种粤香占上为6.427 9,均表现增长趋势;在抗虫品种胜巴丝苗上种群趋势指数达1.513 8,高抗品种Mudgo上仅为0.862 8。其抗虫作用体现在抗虫品种天敌因子的作用远高于感虫品种。测定的水稻主要抗原特征次生物质色谱峰值表明,抗原色谱峰面积大小与抗性水平呈显著负相关性,可用线性回归方程(y=21.076 7–0.597 2x)拟合。
Through seedling resistance determination, resistance of ten rice varieties to brown planthopper （BPH）, Nilaparvata lugens （Stal）, was studied in the green house. Among these rice varieties, special secondary compounds of 6 varieties were characterized by chromatography, and the relationship of the main substance peak in chromatography and the resistance level was analyzed. The variety Mudgo were indentified as highly resistant （HR）, Jingxian89, Huahang8 and Shengbasimiao as resistant （R）, Xiaolongzhan, XianxiaoZhan, Huahangland Yuexiangzhan as moderately resistant （MR）, Shuanggui feeling insect reaction and TN1 as high susceptible （HS） to BPH. The results in green house were confirmed by the population growth index （I） of BPH in the paddy field. I value of BPH population with TN1 and Yuexiangzhan was 28.079 and 6.422 9 respectively, showing an increasing trend of BPH; I value with resistant varieties Mudgo and Shengbasimiao was only 0.862 8 and 1.513 8, respectively. The resistant effects of the resistant varieties caused by natural enemy factors were far more powerful than those of the sensitive varieties. In addition, the content variations of secondary compounds in rice plants indicated that there was significant correlation between compound peak in chromatography and the resistance level. The fit linear regression equation is y=21.076 7-0.597 2x. Therefore, analyzing the secondary compounds by high-performance liquid chromatography （HPLC） could be used as a simple and fast way to identify rice resistance against BPH and predict the dynamics of BPH populations.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第6期626-630,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家科技支撑计划项目(2008BADA5B01
2008BADA5B04)
关键词
水稻
褐飞虱
次生物质
抗性鉴定
rice
brown planthopper
secondary compounds
resistance identification
