摘要
目的:建立双山颗粒HPLC指纹图谱分析方法,科学评价并有效控制双山颗粒剂质量。方法:采用反相高效液相色谱法,色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 mL.min-1,检测波长340 nm,柱温25℃。结果:以绿原酸为参照峰,建立了双山颗粒剂HPLC指纹图谱,确立了13个共有峰,供试品之间的共有峰相似度>0.98。结论:该方法准确,精密度、重复性较好,可作为控制双山颗粒质量的依据。
Objective： HPLC chromatographic fingerprints for Shuangshan granules were developed in order to evaluate the internal quality. Method： HPLC analysis was performed at 25 ℃ by using a Diamonsil C18 column （4.6 mm × 250 mm, 5 μm） , with the gradient elute of mobile phase consisting of acetonitrile-0. 1% glacial acetic acid at the flow rate of 1.0 mL ·min^-1 The UV detection was set at 340 nm. Result： Chlorogenic acid was used as the reference substance in the HPLC chromatographic fingerprint of Shuangshan Granules, which showed 13 common peaks and the similarity was 0. 98 among 10 samples. Conclusion： This method was accurate, repeatable and useful for the quality control of Shuangshan granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期110-112,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西自然科学基金创新研究团队项目(2011GXNSFF018006)
