摘要
目的:采用反相高效液相色谱法(RP-HPLC)建立大青叶的HPLC色谱指纹图谱分析方法,为其品质控制提供可靠依据。方法:Inertsil ODS-3色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸水溶液,梯度洗脱;流速1.0 mL.min-1,检测波长254 nm,柱温30℃。结果:建立了大青叶饮片RP-HPLC标准特征指纹图谱,共确定32个特征指纹峰,其中31个特征峰为共有峰,另一指纹峰可部分检出。结论:建立的大青叶RP-HPLC特征指纹图谱方法专属性强,精密度和重复性良好,为大青叶饮片整体质量控制提供了有效手段。
Objective： RP-HPLC was used to establish a method of fingerprint analysis on Folium Isatidis Pieces. This method can provide a reliable basis for the quality control. Method： Chromatographic column： Inertsil ODS-3 （4. 6 mm ×250 mm, 5 μm） ; mobile phase： ACN-0. 1% formic acid, gradient elution; flow rate： 1.0 mL ^ -1 mm ; detection wavelength： 254 nm; column temperature： 30 ℃. Result： The normal characteristic fingerprint of Folium Isatidis Pieces was established. The result showed that 32 peaks were characteristic including four common peaks and one partially detected peak. Conclusion： With high specificity, precision and good repeatability, the established method provides an effective means for overall quality control of Folium Isatidis Pieces.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期81-85,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药科研专项(201007012-2-11)
