摘要
目的:建立一种简便、灵敏和快速分离测定大鼠脑组织中腐胺、精脒和精胺等多胺含量的高效液相色谱法。方法:采用苯甲酰氯作为衍生化试剂,以1,6-己二胺为内标,采用polygocyl(250×4.6 mm 5μm)C18色谱柱,流动相为甲醇-水(62:38 V/V),流速1.0 mL/min,检测波长229 nm。结果:本法线性范围是1～2 000/mL,回收率大于90%。日内和日间变异系数为1.5%～4.2%。最低定量浓度为1 nmol/mL。结论:该方法符合生物样品分析要求,为脑组织中多胺的含量测定提供了一种简便、准确、灵敏度高的分析方法。
Objective：To develop a simple, sensitive and fast method for determination of putrescine, spermidine and spermine in mouse rat brains. Methods： With the benzoyl chlorides as the derivation reagent and 1,6-Hexanediamine as the internal standard,the polyamines were separated on the polygocyl Cls （250×4.6 mm 5 μm） column using methanol-water（62：38 V/V）as the mobile phase （flow rate 1.0 mL/min） with UV detector at wavelength 229 nm. Results： The standard curve was linear in the content range 1 -2000 nmol·mL^-1. The recoveries were higher than 90% and the RSD was in the range from 1.5% to 4, 2%. The low limits of quantifica- tion were one nmol/mL. Conclusion： The proposed method was suitable for screening polyamines in rat brains.
出处
《川北医学院学报》
CAS
2012年第6期538-541,共4页
Journal of North Sichuan Medical College
基金
河北科技支撑计划资助项目(11230908D-4-2)
关键词
多胺
高效液相色谱
固相萃取
Polyamines
High performance liquid chromatography （HPLC）
Solid-phase extraction（SPE）
