摘要
建立了阿部鲻虾虎鱼(Mugilogobius abei)P糖蛋白(P-gp)mRNA荧光定量RT-PCR检测方法,利用该检测方法探讨不同时间、不同浓度DDT和苯并[a]芘(BaP)暴露对阿部鲻虾虎鱼P-gp基因表达的影响。结果显示,该检测方法能在低质量浓度污染物(0.01 mg·L-1DDT、0.005 mg·L-1BaP)暴露下检测到P-gp基因表达量的显著变化(P<0.05)。P-gp基因在正常阿部鲻虾虎鱼肌肉、肝脏、心脏、肠、脑组织中均有表达,在鳃、脾组织中没有检测到表达,DDT和BaP能诱导肝脏和脑中P-gp基因表达量升高。2种污染物暴露5 h均可诱导肝脏表达量的显著变化,随着暴露时间延长,表达量均升高,24 h达到较高表达量。低质量浓度DDT和BaP均能诱导肝脏P-gp基因表达,并呈现良好的剂量-效应关系,而高质量浓度均抑制P-gp的表达。DDT和BaP可影响阿部鲻虾虎鱼肝脏P-gp基因表达,其变化可作为生物标志物,来评价化学污染物对水生动物的生物学效应。
Persistent Organic Pollutants （POPs） in the environment have become one of severe worldwide problems. The expression of P-glycoprotein （P-gp） in aquatic organisms has been proposed as a biomarker of POPs exposure. The aim of this study was to reveal the effects of DDT and Benzo （a） pyrene （BaP） exposure on expression of p-gp in Mugilogobius abei. The P-gp expression in Mugilogobius abel treated with different concentrations of DDT （0, 0.01, 0.1, 0.4, 2, 10 mg＇L1） and BaP （0, 0.005, 0.025, 0.1, 0.5, 2 mg.L1） for 24 h was determined by quantitative RT-PCR. The results showed that the P-gp mRNA was detected in normal muscle, liver, intestine, heart, whereas no detectable expression was in the gill and spleen. DDT and BaP induced its expression in liver and heart tissue. Both stimulated the expression of P-gp in liver after 5 h exposure, the expression were increased significantly following the exposure time, with a higher expression after 24 h exposure. The significant concentration-effect relationships were detected in low concentration. However, the effects of DDT and BaP in liver tissue was reversed in the high concentration （10, 2 mg.L1, respectively）,. In conclusion, DDT and BaP affects expression of P-gp in Mugilogobius abei, indicating p-gp may be used as the biomarker to assess the biological effects of POPs in aquatic animals.
出处
《生态环境学报》
CSCD
北大核心
2012年第10期1737-1741,共5页
Ecology and Environmental Sciences
基金
国家自然科学基金项目(40976072)
科技部公益项目(2004DIB1J029)
