摘要
目的探讨空气细微颗粒与青年特发性气胸的关系。方法收集武汉大学中南医院胸心外科2011年3月至2011年9月治疗的20例青年特发性气胸患者肺大疱组织标本作为实验组，10例青年正常肺组织标本作为对照组，采用苏木素一伊红（HE）染色法、Wright—Giemsa（W—G）染色法和酶联免疫吸附试验（ELISA）法分别检测肺泡巨噬细胞（AM）、空气细微颗粒及单核细胞趋化蛋白-1（MCP-1）在两组肺组织中的表达。结果实验组可见空气细微颗粒物明显沉积，而对照组肺组织中极少见空气细微颗粒物；实验组AM的百分比为（30．100±3．636）％，对照组为（14．260±4．312）％，AM在实验组表达明显高于对照组（P〈0．05）；实验组肺组织中MCP-1表达水平为（1．191×10^3±184．812）ng／L，对照组为（0．540×10^3±125．058）ng／L，MCP-1在实验组中表达明显增强（P〈0．05）。结论青年特发性气胸肺组织中存在空气细微颗粒，此颗粒刺激气道上皮细胞引发炎性反应，激活AM产生大量的MCP-1，进而促进和加重气道炎性反应，结果提示青年特发性气胸的发生与空气细微颗粒沉积有关。
Objective To observe the relationship between fine particulate matter and idiopathic spontaneous pneumothorax in youngs. Methods Twenty young patients with idiopathic spontaneous pneumothorax as experimental group were randomly collected from March 2011 to September 2011, and ten normal lung tissue specimens served as normal control group. The lung tissue sections stained by hematoxylin and eosin （HE） and Wright-Giemsa （W-G） respectively were examined in terms of alveolar macropbage （AM） and fine particulate matters. The level of MCP-1 in the lung tissue was measured by using enzyme linked immunosorbent assay （ELISA）. Results Fine particulate matters were found obviously in experi- mental group, but rarely seen in normal control group. The percentage of AM in experimental group and normal control group was （30. 100 ＋ 3. 636 ）% and （14. 260 ＋ 4. 312 ）% respectively. MCP-1 levels in serum were （ 1. 191×103 ＋ 184. 812） ng/L and （0. 540× 103 ＋ 125.058） ng/L in experimental group and normal control group respectively. AM counts and MCP-1 levels in experimental group were significantly higher than those in normal control group （P 〈 0. 05 ）. Conclusion In the lung tissues of the idiopathic spontaneous pneumothorax in youngs, there exist fine particulate matters, which can stimulate the airway epithelial cells and cause inflammatory responses. At the same time they can stimulate mononuclear macro- phage to produce amounts of MCP-1, which may contribute to and deteriorate the airway inflammatory responses. It is believed that the deposition of fine particulate matters may be related with the occurrence of idiopathic pontaneous pneumothorax in youngs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第12期2590-2591,F0004,共3页
Chinese Journal of Experimental Surgery
关键词
特发性气胸
空气细微颗粒
肺泡巨噬细胞
单核细胞趋化蛋白-1
Idiopathic pneumothorax
Fine particulate mater
Alveolar macrophage
Mono- cyte chemoattractant protein-1
