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奶牛支原体病的诊断 被引量:10

Diagnosis of Cow Mycoplasmosis
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摘要 2011年,宁夏某牛场奶牛出现以发烧、呼吸困难、严重乳房炎、关节炎为主要特征的病例,病程近3个月。根据临床症状及病理剖检和治疗情况,怀疑为支原体感染。采用支原体分离培养、血清学检查(ELISA)、核酸检测(PCR)及16SRNA序列比对对病原体进行检测。结果表明,该病的病原为牛支原体和无乳支原体混合感染。 In 2011, some cases were found in one cattle farm in Ningxia, where some cows successively took ill characterized by fever, dyspnea, severe mastitis and arthritis. The illness, lasting nearly three months, is suspected as Mycoplasma boris infection according to clinical symptoms, pathological anatomy and treatment. The cases were definitely diagnosed as mixed infection of Mycoplasma boris and Mycoplasrna agalactiae by pathogen isolatiion and culture, serologic test, PCR and 16 S RNA sequence alignment.
出处 《动物医学进展》 CSCD 北大核心 2012年第12期200-204,共5页 Progress In Veterinary Medicine
基金 宁夏科技攻关项目(奶牛疫病防控技术kjnx2009-3)
关键词 奶牛 支原体 酶联免疫吸附试验 聚合酶链反应 基因序列比对 cow Mycoplasma ELASA PCR gene sequence alignment
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二级参考文献8

共引文献77

同被引文献80

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