摘要
分析测定了不同微波灭菌时间对MS培养基的温度、失水率、pH的影响并进行灭菌效果的比较,以期探讨MS培养基微波灭菌效果。结果表明:随着灭菌时间的增加,MS培养基的温度和失水率都随之上升,pH降低,灭菌时间达到2min后,MS培养基彻底灭菌,无杂菌生长;且pH变化很小,营养成分破坏少。
The effect of different microwave sterilizations on the temperature, water loss rate,pH of MS medium were detected,and the sterilization effect was compared to investigate sterilization effect of MS medium. The results showed that with the increase of sterilization time,MS medium temperature and water loss rate rised,pH value reduced. When sterilization time reached 2 min, MS medium was thoroughly sterilization. There was no bacterial growth,and the pH value changed small,the nutrients of MS medium were damaged a little.
出处
《北方园艺》
CAS
北大核心
2012年第23期129-131,共3页
Northern Horticulture
基金
2011年苏州农业职业技术学院院级资助项目(NS1111)
2010年苏州市农业科技支撑计划资助项目(SN201009)
关键词
MS培养基
微波灭菌
PH值
灭菌效果
MS medium
microwave sterilization
pH value
sterilization effect
