摘要
目的:研究两色金鸡菊头状花序的化学成分,为其开发利用奠定化学基础。方法:利用硅胶、Sephadex LH-20、制备液相等色谱方法进行分离,NMR,MS,CD等波谱技术鉴定化合物的结构。结果:分离鉴定了8个化合物,包括有机酸类成分3个,分别为3,4-二羟基苯甲酸(3,4-dihydroxybenzoic acid,1)、4-羟基苯甲酸(4-hydroxybenzoic acid,2)、咖啡酸(caffeic acid,3);有机酸酯类成分1个,为对羟基桂皮酸甲酯葡萄糖苷(4-O-β-D-glucopyranosyl-p-coumaric acid methyl ester,4);二氢黄酮类成分2个,分别为2S-3',5',7-三羟基二氢黄酮(2S-3',5',7-trihydroxyflavanone,5)、(2R,3R)-3,4',5,7-四羟基二氢黄酮[(2R,3R)-3,4',5,7-tetrahydroxylflavanone,6];甾醇类成分2个,分别为豆甾醇葡萄糖苷(stigmasterol-3-O-β-D-glucopyranoside,7)和β-谷甾醇(β-sitosterol,8)。结论:化合物1～7均为首次从该属植物中分离得到,化合物8为首次从该植物中得到。
Objective： To study the chemical constituents of the inflorescences of Coreopsis tinctoria and provide the chemical basis for development and utilization of this plant. Method： Various chromatographic techniques were employed for the isolation and purification of the constituents including silica gel, Sephadex LH-20 and pre-HPLC. The structures of compounds were elucidated by chemical and spectral analysis （ NMR, IR, UV and MS）. Result ： Eight compounds have been isolated from the inflorescences of C. tinctoria, including three organic acids which are 3,4-dihydroxybenzoicacid （1） ,4-hydroxybenzoicacid （2） and caffeic acid （3）, one organic ester which is 4-O-/3-D-glucopyranosyl-p-coumaric acid methyl ester （4）, two flavonones which are 2S-3＇, 5＇, 7-trihydroxyflavanone （ 5 ） and （ 2 R, 3 R ） -3,4＇, 5,7-tetrahydroxylflavanone （ 6 ）, two sterols which are stigmastero1-3-O-β-D-gluc opyranoside （ 7 ） and fl- sitosterol （8）. Conclusion： Compounds 1-7 were isolated from genus Coreopsis for the first time and compound 8 was isolated first from C. tinctoria.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第23期3581-3584,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2012ZX09301002-002-002)
关键词
两色金鸡菊
头状花序
化学成分
有机酸
二氢黄酮
Coreopsis tinctoria
inflorescences
chemical constituents
organic acids
flavanones
