摘要
以HPLC测定不同分子量的标准PEG样品和胆甾醇聚氧乙烯醚(下称cholesterol—(EO)n)的保留值,根据标准PEG聚合度和样品的(EO)n平均加成数(由NMR提供)拟比较样品分子量的粗范围;由HPLC对样品提供的各峰值的面积百分数来设定它的平均加成数方程,并用GFC曲线对应点求解出各峰值加成数。再以此点为对称轴按Poisson分布计算出各峰值的分子量分布范围。
The retentions of standard PEG sample of different molecular weight and cholesterol-(EO)n are determined by HPLC ·Approxi ate ranges of sample molecular weight are compared in term of the polymeric deggee of standard PEG and average addition of E0)n of sample,the eduation of evcrage addition is assumed witho )area percetage of sample peak provided by HPLC.The additions dieach peak areachieved with the opposite dot of GFC curve.Thef qstribution ranges of molecular weight of each peak arecal culated y the distribution of poissoa in this dot for symmetricd axle.
