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Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori 被引量:1

Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori
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摘要 Six sex pheromone synthesis signal genes, including aeyl coenzyme A (acyl- CoA) desaturase (desatl), fatty acyl reduetase (FAR), pheromone biosynthesis activating neuropeptide receptor (PBANR), fatty acid transport protein (FATP), acyl-CoA binding protein (ACBP) and store-operated channel protein (OrailA), were studied for their tran- scriptional regulations. The expression profiles of these transcripts at different develop- mental stages (from -96 to 48 h) revealed that the genes are expressed in an age-dependent manner. The transcripts of these genes continued to increase despite decapitation, and compared with normally developmental females, decapitation significantly inhibited their expression. Further experiments with a methoprene, a juvenile hormone (JH) analogue, challenge showed that JH was not a key inhibiting factor in the expression of these genes, and mating was found to significantly inhibit the expression of these marker genes. Alto- gether, the results provide a reference for understanding the mechanism of sex pheromone synthesis. Six sex pheromone synthesis signal genes, including aeyl coenzyme A (acyl- CoA) desaturase (desatl), fatty acyl reduetase (FAR), pheromone biosynthesis activating neuropeptide receptor (PBANR), fatty acid transport protein (FATP), acyl-CoA binding protein (ACBP) and store-operated channel protein (OrailA), were studied for their tran- scriptional regulations. The expression profiles of these transcripts at different develop- mental stages (from -96 to 48 h) revealed that the genes are expressed in an age-dependent manner. The transcripts of these genes continued to increase despite decapitation, and compared with normally developmental females, decapitation significantly inhibited their expression. Further experiments with a methoprene, a juvenile hormone (JH) analogue, challenge showed that JH was not a key inhibiting factor in the expression of these genes, and mating was found to significantly inhibit the expression of these marker genes. Alto- gether, the results provide a reference for understanding the mechanism of sex pheromone synthesis.
出处 《Insect Science》 CAS CSCD 2012年第6期621-632,共12页 昆虫科学(英文版)
关键词 Bombyx mori sex pheromone biosynthesis signal genes TRANSCRIPTIONALREGULATION Bombyx mori, sex pheromone biosynthesis, signal genes, transcriptionalregulation
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