摘要
目的初步探索肝腺瘤组织细胞所具备的合成、代谢、解毒等肝脏功能活性水平,为其能否成为人工肝新型细胞材料来源提供依据。方法收集近三年来南方医院手术切除并经病理证实为肝腺瘤的组织标本3例,HE染色后电子显微镜下观察其细胞形态及组织结构,并通过糖原染色、免疫组化及qRT-PCR等方法检测肝细胞合成、代谢和转化等功能相关基因的表达情况,同时与正常肝组织细胞的功能水平进行对比。结果肝腺瘤细胞形态与正常肝细胞相似,细胞核无明显异型性,qRT-PCR检测发现肝腺瘤各肝功能相关基因的表达均与正常肝组织细胞相当,部分指标甚至高于正常肝组织细胞。免疫组化检测发现肝腺瘤和正常肝组织中ALB、P450和CK18均有较强表达,AFP除一例肝腺瘤组织有部分表达外余2例和全部正常组织均无表达。结论肝腺瘤细胞有望在解决生物人工肝细胞来源缺乏问题上产生重大突破,有必要进一步开展肝腺瘤的建株研究。
Objective To provide the evidence for hepatocellular adenoma as the ideal cell source of bioartificial liver by exploring the synthesis,metabolism and detoxification function of hepatocellular adenoma.Methods We collected three surgical resection specimens during past two years in Nanfang Hospital.The specimens were pathologically confirmed as hepatic adenoma.The functionally related genes were detected by immunohistochemistry and qRT-PCR.The results were compared with normal liver tissue.Results Necleolus of hepatocellular adenoma was similar to normal liver tissue in cell morphology.The expressions of ALB,P450 and CK18 were high in both hepatocellular adenoma and normal tissue,but AFP was not expressed in hepatocellular adenoma and normal tissue except one hepatocellular adenoma tissue.QRT-PCR showed that the functionally related genes were all high in hepatocellular adenoma,even higher than normal tissues,but the expressions of AFP in hepatocellular adenoma were lower than normal tissue except one.Conclusion Hepatocellular adenoma was close to normal liver tissue both in cell morphology and in a variety of functional proteins and enzymes,and was expected to become the ideal cell source for artificial liver biological materials.
出处
《现代消化及介入诊疗》
2012年第5期251-255,共5页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
广东省教育部产学研结合项目(2011B090400018)
广东省科技计划项目(2009B060300020)
广东省高等学校人才引进专项资金项目(粤财教字[2011]431号)
关键词
肝腺瘤
生物人工肝
生物材料
肝细胞功能
Hepatocellular adenoma
Bioartificial liver
biological materials
Liver cell function
