摘要
利用短期诱导实验 ,分析了花生四烯酸对红豆杉细胞培养中紫杉烷合成的影响。在适宜浓度的花生四烯酸诱导下 ,紫杉醇 (taxol)的产量提高了近 3倍 ,同时 10 -去乙酰基巴卡亭 (10 - DAB)与巴卡亭 (baccatin )相应上升。通过对紫杉醇合成代谢途径的等拓扑结构简化与动力学分析 ,获得了诱导子加入后可能的 3种中间代谢产物浓度转移模式。结合花生四烯酸短期诱导实验结果 ,提出了花生四烯酸的加入提高了 10 - DAB合成通量的观点 。
The influence of arachidonic acid on the production of taxanes in the process of plant cell culture was studied by short term elicitation experiments Experiments showed that arachidonic acid at a concentration of 0 1 mg/L can improve the production of taxol nearly three fold It was discovered that the concentration of 10 deacetyl baccatin Ⅲ (10 DAB Ⅲ) and baccatin Ⅲ were also increased while taxol concentration increases under arachidonic acid elicitation By dynamic kinetic analysis on the simplified taxol biosynthetic pathway, the mode of taxoid concentration transformation may proceed in three different ways On the basis of experimental results of arachidonic acid elicitation, it could be postulated that at certain concentration of arachidonic acid the reaction rate for the production of 10 DAB Ⅲ could be increased To define such a point precisely may provide a basis to achieve a synergistic action of multiple elicitors
出处
《中草药》
CAS
CSCD
北大核心
2000年第6期418-421,共4页
Chinese Traditional and Herbal Drugs
基金
教育部"跨世纪优秀人才培养计划"基金
天津市科委项目! ( 963 10 72 11)
关键词
紫杉醇
花生四烯酸
作用位点
生物合成
taxol arachidonic acid the acting point of enzyme plant cell culture
