摘要
通过分析已公布的658个随机挑选的SSR标记在12个高粱属材料中的多态性信息,发现基因中内含子标记较非内含子标记有更高的多态性,因此,本研究利用生物信息学方法进行了高粱全基因组内含子标记的开发。首先,对高粱29448条CDS序列进行内含子位点挖掘,找到115545个内含子位点。其次,在内含子两侧的序列上设计引物,经e-PCR在高粱基因组上筛选及定位,共获得82742个单拷贝内含子标记。其在染色体上数量变异范围为3794个(5号染色体)～15371个(1号染色体)之间,平均每条染色体上8237个。此外还构建了1个公共数据库(www.sicau.edu.cn/web/yms/sip/SIP.html),以便研究人员下载及使用。
The intron markers were found to be more polymorphic than the non-intron ones within genes through our analysis of the published polymorphism information of the 658 randomly selected SSR markers in 12 lines of Sorghum spp.Therefore,genome-wide intron polymorphism markers in sorghum bicolor were developed via bioinformatics approaches.115545 introns were found through analyzing the 29448 CDS sequences of Sorghum bicolor,then,primers were designed in the flanking sequences of the introns,and were filtered and mapped in Sorghum bicolor genome with the e-PCR program.82742 intron markers with single copy were obtained finally.The number of markers per chromosome ranged from 3794（chromosome 5） to 15371（chromosome 1）,with an average of 8237.A web-based database named SIP was established to provide all related information for the convenient use of the markers,which is available at http：//www.sicau.edu.cn/web/yms/sip/SIP.html.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第8期1101-1105,共5页
Journal of Nuclear Agricultural Sciences
基金
四川省教育厅重点项目(11ZA088)
关键词
高粱
内含子标记
SSR标记
遗传连锁图谱
Sorghum bicolor
intron polymorphism marker
SSR marker
genetic linkage map
